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DNA Ligase 4 (LIG4) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS951845

This protein is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining… More details

$940 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA ligase 4 (LIG4), Recombinant Protein
Full Product Name
Recombinant Human DNA ligase 4 (LIG4) , partial
Product Gene Name
LIG4 recombinant protein
Sequence
KISNIFEDVE FCVMSGTDSQ PKPDLENRIA EFGGYIVQNP GPDTYCVIAG SENIRVKNII LSNKHDVVKP AWLLECFKTK SFVPWQPRFM IHMCPSTKEH FAREYDCYGD SYFIDTDLNQ LKEVFSGIKN SNEQTPEEMA SLIADLEYRY SWDCSPLSMF RRHTVYLDSY AVINDLSTKN EGTRLAIKAL ELRFHGAKVV SCLAEGVSHV IIGEDHSRVA DFKAFRRTFK RKFKILKESW VTDSIDKCEL QEENQYLI
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
103,971 Da
Sequence Positions
654-911aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
601837
3D Structure
ModBase 3D Structure for P49917
Species
Human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LIG4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIG4 purchase

MBS951845 is a recombinant protein targeting LIG4. Also known as Recombinant Human DNA ligase 4 (LIG4) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for LIG4. It may not necessarily be applicable to this product.

NCBI GI #
148539894
NCBI GeneID
3981
NCBI Accession #
NP_001091738.1
UniProt Primary Accession #
P49917
UniProt Secondary Accession #
Q8IY66; Q8TEU5
UniProt Related Accession #
P49917
GenBank Nucleotide #
NM_001098268.1
NCBI Official Full Name
DNA ligase 4 isoform 1
NCBI Official Synonym Full Names
DNA ligase 4
NCBI Official Symbol
LIG4
NCBI Official Synonym Symbols
LIG4S
UniProt Protein Name
DNA ligase 4
UniProt Synonym Protein Names
DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Protein Family
DNA ligase
UniProt Gene Name
LIG4
NCBI Protein Information
DNA ligase 4

NCBI Summary for LIG4

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for LIG4

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
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Research Articles on LIG4

1. This study shown that both ligase IV and XRCC4 may act in concert to modulate the development of glioma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIG4 recombinant protein

 Reference Product  PubMed Publications
 XRCC4 recombinant protein  >41 publications with LIG4 and XRCC4
 PRKDC recombinant protein  >12 publications with LIG4 and PRKDC
 NHEJ1 recombinant protein  >5 publications with LIG4 and NHEJ1
 APLF recombinant protein  >1 publications with LIG4 and APLF

Pathways associated with LIG4 recombinant protein

 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Integration Of Provirus Pathway antibodies  Integration Of Provirus Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram

Diseases associated with LIG4 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >42 publications with LIG4 and Neoplasms
 LIG4 Syndrome Antibodies  >27 publications with LIG4 and LIG4 Syndrome
 Nervous System Diseases Antibodies  >10 publications with LIG4 and Nervous System Diseases
 Lymphoma Antibodies  >9 publications with LIG4 and Lymphoma
 Respiratory Tract Diseases Antibodies  >6 publications with LIG4 and Respiratory Tract Diseases
 Neoplasms, Experimental Antibodies  >6 publications with LIG4 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >5 publications with LIG4 and Drug Toxicity
 Carcinoma, Squamous Cell Antibodies  >5 publications with LIG4 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >4 publications with LIG4 and Lung Neoplasms
 Disease Models, Animal Antibodies  >4 publications with LIG4 and Disease Models, Animal

Organs/Tissues associated with LIG4 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with LIG4 and Blood
 Brain Antibodies  >8 publications with LIG4 and Brain
 Bone Antibodies  >7 publications with LIG4 and Bone
 Skin Antibodies  >4 publications with LIG4 and Skin
 Prostate Antibodies  >3 publications with LIG4 and Prostate
 Muscle Antibodies  >2 publications with LIG4 and Muscle
 Pharynx Antibodies  >2 publications with LIG4 and Pharynx
 Thymus Antibodies  >2 publications with LIG4 and Thymus
 Kidney Antibodies  >1 publications with LIG4 and Kidney
 Testis Antibodies  >1 publications with LIG4 and Testis
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