• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Glycogen Phosphorylase (PYGL), Partial (PYGL) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS952730

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glycogen phosphorylase (PYGL), partial, Recombinant Protein
Full Product Name
Recombinant Human Glycogen phosphorylase, liver form (PYGL), partial
Product Gene Name
Sequence
AKPLTDQEKR RQISIRGIVG VENVAELKKS FNRHLHFTLV KDRNVATTRD YYFALAHTVR DHLVGRWIRT QQHYYDKCPK RVYYLSLEFY MGRTLQNTMI NLGLQNACDE AIYQLGLDIE ELEEIEEDAG LGNGGLGRLA ACFLDSMATL GLAAYGYGIR YEYGIFNQKI RDGWQVEEAD DWLRYGNPWE KSRPEFMLPV HFYGKVEHTN TGTKWIDTQV VLALPYDTPV PGYMNNTVNT MRLWSARAPN DFNLRDFNVG DYIQAVLDRN LAENISRVLY PNDNFFEGKE LRLKQEYFVV AATLQDIIRR FKASKFGSTR GAGTVFDAFP DQVAIQLNDT HPALAIPELM RIFVDIEKLP WSKAWELTQK TFAYTNHTVL PEALERWPVD LVEKLLPRHL EIIYEINQKH LDRIVALFPK DVDRLRRMSL IEEEGSKRIN MAHLCIVGSH AVNGVAKIHS DIVKTKVFKD FSELEPDKFQ NKTNGITPRR WLLLCNPGLA ELIAEKIGED YVKDLSQLTK LHSFLGDDVF LRELAKVKQE NKLKFSQFLE TEYKVKINPS SMFDVQVKRI HEYKRQLLNC LHVITMYNRI KKDPKKLFVP RTVIIGGKAA PGYHMAKMII KLITSVADVV NNDPMVGSKL KVIFLENYRV SLAEKVIPAT DLSEQISTAG TEASGTGNMK FMLNGALTIG TMDGANVEMA EEAGEENLFI FGMRIDDVAA LDKKGYEAKE YYEALPELKL VIDQIDNGFF SPKQPDLFKD IINMLFYHDR FKVFADYEAY VKCQDKVSQL YMNPKAWNTM VLKNIAASGK FSSDRTIKEY AQNIWNVEPS DLKISLSNES NKVNG
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
123.9 kDa
Sequence Positions
2-846. Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
232700
3D Structure
ModBase 3D Structure for P06737

Product Categories/Family for PYGL recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for PYGL recombinant protein

Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage.Newgard C.B., Nakano K., Hwang P.K., Fletterick R.J.Proc. Natl. Acad. Sci. U.S.A. 83:8132-8136(1986) Carty M.D., Clancy Y.C., Soeller W.C. Identification of a mutation in liver glycogen phosphorylase in glycogen storage disease type VI.Chang S., Rosenberg M.J., Morton H., Francomano C.A., Biesecker L.G.Hum. Mol. Genet. 7:865-870(1998) Mutations in the liver glycogen phosphorylase gene 'PYGL' underlying glycogenosis type VI.Burwinkel B., Bakker H.D., Herschkovitz E., Moses S.W., Shin Y.S., Kilimann M.W.Am. J. Hum. Genet. 62:785-791(1998) Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) The DNA sequence and analysis of human chromosome 14.Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.Nature 421:601-607(2003)

Other Notes

Small volumes of PYGL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PYGL purchase

MBS952730 is a recombinant protein targeting PYGL. Also known as Recombinant Human Glycogen phosphorylase, liver form (PYGL), partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for PYGL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glycogen phosphorylase, liver form isoform 2
NCBI Official Synonym Full Names
phosphorylase, glycogen, liver
NCBI Official Symbol
PYGL
NCBI Official Synonym Symbols
GSD6
UniProt Protein Name
Glycogen phosphorylase, liver form
UniProt Gene Name
PYGL
UniProt Entry Name
PYGL_HUMAN
NCBI Protein Information
glycogen phosphorylase, liver form

NCBI Summary for PYGL

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]
Show more

UniProt Comments for PYGL

PYGL: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. Belongs to the glycogen phosphorylase family.

Protein type: Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1; Transferase

Chromosomal Location of Human Ortholog: 14q21-q22

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: AMP binding; ATP binding; bile acid binding; drug binding; glucose binding; glycogen phosphorylase activity; protein binding; protein homodimerization activity; purine binding; pyridoxal phosphate binding; vitamin binding

Biological Process: 5-phosphoribose 1-diphosphate biosynthetic process; carbohydrate metabolic process; glucose homeostasis; glucose metabolic process; glycogen catabolic process; glycogen metabolic process

Disease: Glycogen Storage Disease Vi
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000