Interleukin-4 (IL4) Human Recombinant Protein

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.Yokota T., Otsuka T., Mosmann T., Banchereau J., Defrance T., Blanchard D., de Vries J.E., Lee F., Arai K.Proc. Natl. Acad. Sci. U.S.A. 83:5894-5898(1986) Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression.Arai N., Nomura D., Villaret D., Malefijt R.D., Seiki M., Yoshida M., Minoshima S., Fukuyama R., Maekawa M., Kudoh J., Shimizu N., Yokota K., Abe E., Yokota T., Takebe Y., Arai K.J. Immunol. 142:274-282(1989) An alternatively spliced interleukin 4 form in lymphoid cells.Klein S.C., Golverdingen J., Bouwens A.G.M., Tilanus M.G.J.Immunogenetics 41:57-57(1995) SeattleSNPs variation discovery resource The 5' region of the human interleukin 4 gene structure and potential regulatory elements.Eder A., Krafft-Czepa H., Krammer P.H.Nucleic Acids Res. 16:772-772(1988) Disulfide assignments in recombinant mouse and human interleukin 4.Carr C., Aykent S., Kimack N.M., Levine A.D.Biochemistry 30:1515-1523(1991) Crystal structure of recombinant human interleukin-4.Walter M.R., Cook W.J., Zhao B.G., Cameron R.P. Jr., Ealick S.E., Walter R.L. Jr., Reichert P., Nagabhushan T.L., Trotta P.P., Bugg C.E.J. Biol. Chem. 267:20371-20376(1992) Crystal structure of human recombinant interleukin-4 at 2.25-A resolution.Wlodaver A., Pavlovsky A., Gustchina A.FEBS Lett. 309:59-64(1992) Crystal structure of the interleukin-4/receptor alpha chain complex reveals a mosaic binding interface.Hage T., Sebald W., Reinemer P.Cell 97:271-281(1999) Secondary structure and topology of human interleukin 4 in solution.Redfield C., Smith L.J., Boyd J., Lawrence G.M.P., Edwards R.G., Smith R.A.G., Dobson C.M.Biochemistry 30:11029-11035(1991) Human interleukin 4. The solution structure of a four-helix bundle protein.Smith L.J., Redfield C., Boyd J., Lawrence G.M.P., Edwards R.G., Smith R.A.G., Dobson C.M.J. Mol. Biol. 224:899-904(1992) 1H, 15N, 13C, and 13CO assignments of human interleukin-4 using three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy.Powers R., Garret D.S., March C.J., Frieden E.A., Gronenborn A.M., Clore G.M.Biochemistry 31:4334-4346(1992) Determination of the secondary structure and folding topology of human interleukin-4 using three-dimensional heteronuclear magnetic resonance spectroscopy.Garret D.S., Powers R., March C.J., Frieden E.A., Clore G.M., Gronenborn A.M.Biochemistry 31:4347-4353(1992) Three-dimensional solution structure of human interleukin-4 by multidimensional heteronuclear magnetic resonance spectroscopy.Powers R., Garret D.S., March C.J., Frieden E.A., Gronenborn A.M., Clore G.M.Science 256:1673-1677(1992) Experimental and theoretical studies of the three-dimensional structure of human interleukin-4.Curtis B.M., Presnell S.R., Srinivasan S., Sassenfeld H., Klinke R., Jeffery E., Cosman D., March C.J., Cohen F.E.Proteins 11:111-119(1991) Aspects of receptor binding and signalling of interleukin-4 investigated by site-directed mutagenesis and NMR spectroscopy.Mueller T., Dieckmann T., Sebald W., Oschkinat H.J. Mol. Biol. 237:423-436(1994) Comparison of four independently determined structures of human recombinant interleukin-4.Smith L.J., Redfield C., Smith R.A.G., Dobson C.M., Clore G.M., Gronenborn A.M., Walter M.R., Naganbushan T.L., Wlodawer A.Nat. Struct. Biol. 1:301-310(1994) Polymorphism in the P-selectin and interleukin-4 genes as determinants of stroke a population-based, prospective genetic analysis.Zee R.Y.L., Cook N.R., Cheng S., Reynolds R., Erlich H.A., Lindpaintner K., Ridker P.M.Hum. Mol. Genet. 13:389-396(2004)

Small volumes of IL4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS955105 is a recombinant protein targeting IL4. Also known as Recombinant Human Interleukin-4. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.