• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

General Transcription Factor IIF Subunit 2 (GTF2F2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS955936

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. More details

$385 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
General transcription factor IIF subunit 2 (GTF2F2), Recombinant Protein
Full Product Name
Recombinant Human General transcription factor IIF subunit 2
Product Gene Name
GTF2F2 recombinant protein
Product Synonym Names
ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30
Sequence
AERGELDLTG AKQNTGVWLV KVPKYLSQQW AKASGRGEVG KLRIAKTQGR TEVSFTLNED LANIHDIGGK PASVSAPREH PFVLQSVGGQ TLTVFTESSS DKLSLEGIVV QRAECRPAAS ENYMRLKRLQ IEESSKPVRL SQQLDKVVTT NYKPVANHQY NIEYERKKKE DGKRARADKQ HVLDMLFSAF EKHQYYNLKD LVDITKQPVV YLKEILKEIG VQNVKGIHKN TWELKPEYRH YQGEEKSD
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
44.2 kDa
Sequence Positions
2-249aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
189969
3D Structure
ModBase 3D Structure for P13984

Product Categories/Family for GTF2F2 recombinant protein

Transcription
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-PAGE of GTF2F2 recombinant protein

Human GTF2F2 Recombinant Protein – Cat# MBS955936

Product References and Citations for GTF2F2 recombinant protein

Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.Sopta M., Burton Z.F., Greenblatt J.Nature 341:410-414(1989)

Other Notes

Small volumes of GTF2F2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2F2 purchase

MBS955936 is a recombinant protein targeting GTF2F2. Also known as Recombinant Human General transcription factor IIF subunit 2. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2F2. It may not necessarily be applicable to this product.

NCBI GI #
4758488
NCBI GeneID
2963
NCBI Accession #
NP_004119.1
UniProt Primary Accession #
P13984
UniProt Secondary Accession #
Q5W0H3; A6NNS5
UniProt Related Accession #
P13984
GenBank Nucleotide #
NM_004128.2
NCBI Official Full Name
general transcription factor IIF subunit 2
NCBI Official Synonym Full Names
general transcription factor IIF subunit 2
NCBI Official Symbol
GTF2F2
NCBI Official Synonym Symbols
BTF4; RAP30; TF2F2; TFIIF
UniProt Protein Name
General transcription factor IIF subunit 2
UniProt Synonym Protein Names
ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30
Protein Family
General transcription factor
UniProt Gene Name
GTF2F2
UniProt Synonym Gene Names
RAP30; TFIIF-beta
UniProt Entry Name
T2FB_HUMAN
NCBI Protein Information
general transcription factor IIF subunit 2

UniProt Comments for GTF2F2

GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family.

Protein type: Helicase; Transcription initiation complex; EC 3.6.4.12; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: holo TFIIH complex; microtubule cytoskeleton; nucleoplasm; nucleus; transcription factor TFIIF complex

Molecular Function: ATP binding; DNA binding; helicase activity; protein binding

Biological Process: gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcriptional preinitiation complex assembly; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA splicing; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction
Show more

Research Articles on GTF2F2

1. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GTF2F2 recombinant protein

 Reference Product  PubMed Publications
 GTF2F1 recombinant protein  >2 publications with GTF2F2 and GTF2F1
 GTF2B recombinant protein  >1 publications with GTF2F2 and GTF2B

Pathways associated with GTF2F2 recombinant protein

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram

Organs/Tissues associated with GTF2F2 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >1 publications with GTF2F2 and Mammary Gland
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000