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DNA-Dependent Protein Kinase Catalytic Subunit (PRKDC) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS956945

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties.… More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DNA-dependent protein kinase catalytic subunit (PRKDC), Recombinant Protein
Full Product Name
Recombinant Mouse DNA-dependent protein kinase catalytic subunit
Product Gene Name
Product Synonym Gene Name
HYRC; HYRC1
Product Synonym Names
p460
Sequence
EYPFLVKGGE DLRQDQRIEQ IFEVMNAILS QDAACSQRNM QLRTYRVVPM TSRLGLIEWI ENTMTLKDLL LSNMSQEEKV ANNSDPKAPI RDYKDWLMKV SGKSDAGAYV LMYSRANRTE TVVAFRRRES QVPPDLLKRA FVKMSTSPEA FLALRSHFAS SHALLCISHW LLGIGDRHLN NFMVAMETGS VIGIDFGHAF GSATQFLPVP ELMPFRLTRQ FVSLMLPMKE TGLMCTVMVH ALRAFRSCAG LLTDTMEIFV KEPSFDWKS
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
34.6 kDa
Sequence Positions
3747-4015aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P97313
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for PRKDC recombinant protein

Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.Araki R., Fujimori A., Hamatani K., Mita K., Saito T., Mori M., Fukumura R., Morimyo M., Muto M., Itoh M., Tatsumi K., Abe M.Proc. Natl. Acad. Sci. U.S.A. 94:2438-2443(1997) Murine cell line SX9 bearing a mutation in the DNA-PKcs gene exhibits aberrant V(D) J recombination not only in the coding joint but also in the signal joint.Fukumura R., Araki R., Fujimori A., Mori M., Saito T., Watanabe F., Sarashi M., Itsukaichi H., Eguch-Kasai K., Sato K., Tatsumi K., Abe M.J. Biol. Chem. 273:13058-13064(1998) The murine DNA-PKcs gene consists of 86 exons dispersed in more than 250 kb.Fujimori A., Araki R., Fukumura R., Saito T., Mori M., Mita K., Tatsumi K., Abe M.Genomics 45:194-199(1997) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) Mouse Cdc21 only 0.5 kb upstream from DNA-PKcs in a head-to-head organization an implication of co-evolution of ATM family members and cell cycle regulating genes.Saito T., Matsuda Y., Ishii H., Watanabe F., Mori M., Hayashi A., Araki R., Fujimori A., Fukumura R., Morimyo M., Tatsumi K., Hori T., Abe M.Mamm. Genome 9:769-772(1998) Nonhomologous end-joining deficiency of L5178Y-S cells is not associated with mutation in the ABCDE autophosphorylation cluster.Brzoska K., Kruszewski M., Szumiel I.Acta Biochim. Pol. 53:233-236(2006) Cloning and chromosomal mapping of the mouse DNA-dependent protein kinase gene.Hamatani K., Matsuda Y., Araki R., Itoh M., Abe M.Immunogenetics 45:1-5(1996) Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.Blunt T., Gell D., Fox M., Taccioli G.E., Lehmann A.R., Jackson S.P., Jeggo P.A.Proc. Natl. Acad. Sci. U.S.A. 93:10285-10290(1996) Biochemical and genetic defects in the DNA-dependent protein kinase in murine scid lymphocytes.Danska J.S., Holland D.P., Mariathasan S., Williams K.M., Guidos C.J.Mol. Cell. Biol. 16:5507-5517(1996) Functional interaction between DNA-PK and c-Abl in response to DNA damage.Kharbanda S., Pandey P., Jin S., Inoue S., Bharti A., Yuan Z.-M., Weichselbaum R., Weaver D., Kufe D.Nature 386:732-735(1997) Functional interaction between DNA-PKcs and telomerase in telomere length maintenance.Espejel S., Franco S., Sgura A., Gae D., Bailey S.M., Taccioli G.E., Blasco M.A.EMBO J. 21:6275-6287(2002) GAPDH mediates nitrosylation of nuclear proteins.Kornberg M.D., Sen N., Hara M.R., Juluri K.R., Nguyen J.V., Snowman A.M., Law L., Hester L.D., Snyder S.H.Nat. Cell Biol. 12:1094-1100(2010) Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length.Gao P., Yoo S.H., Lee K.J., Rosensweig C., Takahashi J.S., Chen B.P., Green C.B.J. Biol. Chem. 288:35277-35286(2013)

Other Notes

Small volumes of PRKDC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKDC purchase

MBS956945 is a recombinant protein targeting PRKDC. Also known as Recombinant Mouse DNA-dependent protein kinase catalytic subunit. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKDC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit
NCBI Official Synonym Full Names
protein kinase, DNA activated, catalytic polypeptide
NCBI Official Symbol
Prkdc
NCBI Official Synonym Symbols
p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; AI326420; AU019811; DNA-PKcs
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Synonym Protein Names
p460
UniProt Gene Name
Prkdc
UniProt Synonym Gene Names
Xrcc7; DNA-PK catalytic subunit; DNA-PKcs
UniProt Entry Name
PRKDC_MOUSE
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit

UniProt Comments for PRKDC

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; EC 2.7.11.1; DNA repair, damage; ATYPICAL group; PIKK family; DNAPK subfamily

Cellular Component: DNA-dependent protein kinase complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleus; transcription factor complex

Molecular Function: ATP binding; DNA binding; DNA-dependent protein kinase activity; double-stranded DNA binding; enzyme binding; kinase activity; nucleotide binding; phosphotransferase activity, alcohol group as acceptor; protein binding; protein kinase activity; protein serine/threonine kinase activity; transcription factor binding; transferase activity

Biological Process: B cell lineage commitment; brain development; cell proliferation; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; germ cell programmed cell death; heart development; immunoglobulin production; immunoglobulin V(D)J recombination; lymphocyte differentiation; negative regulation of apoptosis; negative regulation of immunoglobulin production; negative regulation of protein amino acid phosphorylation; peptidyl-serine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of developmental growth; positive regulation of fibroblast proliferation; positive regulation of immune system process; positive regulation of transcription from RNA polymerase II promoter; pro-B cell differentiation; protein destabilization; regulation of circadian rhythm; regulation of smooth muscle cell proliferation; response to DNA damage stimulus; response to gamma radiation; response to ionizing radiation; rhythmic process; somitogenesis; spleen development; T cell differentiation in the thymus; T cell lineage commitment; T cell receptor V(D)J recombination; telomere capping; telomere maintenance; thymus development; V(D)J recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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