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Wilms Tumor Protein Homolog (WT1) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS957212

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a prolineglutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated… More details

$455 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Wilms tumor protein homolog (Wt1), Recombinant Protein
Full Product Name
Recombinant Mouse Wilms tumor protein homolog (Wt1)
Product Gene Name
Wt1 recombinant protein
Sequence
MGSDVRDLNA LLPAVSSLGG GGGGCGLPVS GARQWAPVLD FAPPGASAYG SLGGPAPPPA PPPPPPPPHS FIKQEPSWGG AEPHEEQCLS AFTLHFSGQF TGTAGACRYG PFGPPPPSQA SSGQARMFPN APYLPSCLES QPTIRNQGYS TVTFDGAPSY GHTPSHHAAQ FPNHSFKHED PMGQQGSLGE QQYSVPPPVY GCHTPTDSCT GSQALLLRTP YSSDNLYQMT SQLECMTWNQ MNLGATLKGM AAGSSSSVKW TEGQSNHGIG YESENHTAPI LCGAQYRIHT HGVFRGIQDV RRVSGVAPTL VRSASETSEK RPFMCAYPGC NKRYFKLSHL QMHSRKHTGE KPYQCDFKDC ERRFSRSDQL KRHQRRHTGV KPFQCKTCQR KFSRSDHLKT HTRTHTGKTS EKPFSCRWHS CQKKFARSDE LVRHHNMHQR NMTKLHVAL
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
54,943 Da
Sequence Positions
1-449aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P22561
Species
Mouse
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-PAGE of Wt1 recombinant protein

Mouse WT1 Recombinant Protein – Cat# MBS957212

Other Notes

Small volumes of Wt1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Wt1 purchase

MBS957212 is a recombinant protein targeting Wt1. Also known as Recombinant Mouse Wilms tumor protein homolog (Wt1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Wt1. It may not necessarily be applicable to this product.

NCBI GI #
139779
NCBI GeneID
22431
NCBI Accession #
P22561.1
UniProt Primary Accession #
P22561
UniProt Secondary Accession #
A2A402
UniProt Related Accession #
P22561
NCBI Official Full Name
Wilms tumor protein homolog
NCBI Official Synonym Full Names
Wilms tumor 1 homolog
NCBI Official Symbol
Wt1
NCBI Official Synonym Symbols
Wt-1; D630046I19Rik
UniProt Protein Name
Wilms tumor protein homolog
Protein Family
Wilms tumor protein
UniProt Gene Name
Wt1
UniProt Synonym Gene Names
Wt-1
NCBI Protein Information
Wilms tumor protein homolog

NCBI Summary for Wt1

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013]
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UniProt Comments for Wt1

Transcription factor that plays an important role in cellular development and cell survival (PubMed:16467207, PubMed:16920711, PubMed:17537799). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (). Regulates the expression of numerous target genes, including EPO (PubMed:16467207). Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:17167543). Isoform 1 has lower affinity for DNA, and can bind RNA.
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Research Articles on Wt1

1. both the local oxygen environment and WT1, which enhances KDR expression, contribute to sex-specific Sox9 expression in developing murine gonads

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Wt1 recombinant protein

 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with Wt1 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1641 publications with Wt1 and Neoplasms
 Wilms Tumor Antibodies  >1554 publications with Wt1 and Wilms Tumor
 Kidney Diseases Antibodies  >769 publications with Wt1 and Kidney Diseases
 Leukemia, Myeloid Antibodies  >378 publications with Wt1 and Leukemia, Myeloid
 Leukemia, Myeloid, Acute Antibodies  >315 publications with Wt1 and Leukemia, Myeloid, Acute
 Carcinoma Antibodies  >291 publications with Wt1 and Carcinoma
 Chromosome Aberrations Antibodies  >179 publications with Wt1 and Chromosome Aberrations
 Denys-Drash Syndrome Antibodies  >173 publications with Wt1 and Denys-Drash Syndrome
 Hematologic Diseases Antibodies  >165 publications with Wt1 and Hematologic Diseases
 Adenocarcinoma Antibodies  >156 publications with Wt1 and Adenocarcinoma

Organs/Tissues associated with Wt1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >830 publications with Wt1 and Kidney
 Blood Antibodies  >456 publications with Wt1 and Blood
 Testis Antibodies  >135 publications with Wt1 and Testis
 Embryonic Tissue Antibodies  >123 publications with Wt1 and Embryonic Tissue
 Lung Antibodies  >118 publications with Wt1 and Lung
 Ovary Antibodies  >112 publications with Wt1 and Ovary
 Heart Antibodies  >77 publications with Wt1 and Heart
 Brain Antibodies  >76 publications with Wt1 and Brain
 Uterus Antibodies  >42 publications with Wt1 and Uterus
 Pancreas Antibodies  >11 publications with Wt1 and Pancreas
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