Neutrophil Elastase (ELANE) Human Recombinant Protein

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Nucleotide sequence of human bone marrow serine protease (medullasin) gene.Nakamura H., Okano K., Aoki Y., Shimizu H., Naruto M.Nucleic Acids Res. 15:9601-9601(1987) Structure of the human neutrophil elastase gene.Takahashi H., Nukiwa T., Yoshimura K., Quick C.D., States D.J., Holmes M.D., Whang-Peng J., Knutsen T., Crystal R.G.J. Biol. Chem. 263:14739-14747(1988) The human neutrophil elastase gene. Analysis of the nucleotide sequence reveals three distinct classes of repetitive DNA.Farley D., Travis J., Salvesen G.Biol. Chem. Hoppe-Seyler 370:737-744(1989) Functional expression of human leukocyte elastase (HLE) /medullasin in eukaryotic cells.Okano K., Aoki Y., Shimizu H., Naruto M.Biochem. Biophys. Res. Commun. 167:1326-1332(1990) NIEHS SNPs program Molecular cloning of complementary DNA for human medullasin an inflammatory serine protease in bone marrow cells.Okano K., Aoki Y., Sakurai T., Kajitani M., Kanai S., Shimazu T., Shimizu H., Naruto M.J. Biochem. 102:13-16(1987) Primary structure of human neutrophil elastase.Sinha S., Watorek W., Karr S., Giles J., Bode W., Travis J.Proc. Natl. Acad. Sci. U.S.A. 84:2228-2232(1987) Travis J., Giles P.J., Porcelli L., Reilly C.F., Baugh R., Powers J.(In) Protein degradation in health and disease, Ciba Foundation Symposium, pp.75:51-68, Excerpta Medica, Amsterdam and Oxford (1980) Antibiotic proteins of human polymorphonuclear leukocytes.Gabay J.E., Scott R.W., Campanelli D., Griffith J., Wilde C., Marra M.N., Seeger M., Nathan C.F.Proc. Natl. Acad. Sci. U.S.A. 86:5610-5614(1989) Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis.Gaskin G., Kendal H., Coulthart A., Turner N., Pusey C.D.J. Immunol. Methods 180:25-33(1995) Myelomonocytic cell lineage expression of the neutrophil elastase gene.Takahashi H., Nukiwa T., Basset P., Cystal R.G.J. Biol. Chem. 263:2543-2547(1988) Molecular cloning of human neutrophil elastase.Farley D., Salvesen G.S., Travis J.Biol. Chem. Hoppe-Seyler 369:3-7(1988) The primary structure and elastinolytic activity of medullasin (a serine protease of bone marrow) .Aoki Y., Hase T.Biochem. Biophys. Res. Commun. 178:501-506(1991) Human leukocyte elastase and cathepsin G are specific inhibitors of C5a-dependent neutrophil enzyme release and chemotaxis.Tralau T., Meyer-Hoffert U., Schroder J.M., Wiedow O.Exp. Dermatol. 13:316-325(2004) A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia.Duan Z., Li F.-Q., Wechsler J., Meade-White K., Williams K., Benson K.F., Horwitz M.Mol. Cell. Biol. 24:58-70(2004) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution.Navia M.A., McKeever B.M., Springer J.P., Lin T.-Y., Williams H.R., Fluder E.M., Dorn C.P., Hoogsteen K.Proc. Natl. Acad. Sci. U.S.A. 86:7-11(1989) The refined 2.3-A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor.Wei A.-Z., Mayr I., Bode W.FEBS Lett. 234:367-373(1988) X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor.Bode W., Wei A.-Z., Huber R., Meyer E., Travis J., Neumann S.EMBO J. 5:2453-2458(1986) Mutations in ELA2, encoding neutrophil elastase, define a 21-day biological clock in cyclic haematopoiesis.Horwitz M., Benson K.F., Person R.E., Aprikyan A.G., Dale D.C.Nat. Genet. 23:433-436(1999) Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia.Dale D.C., Person R.E., Bolyard A.A., Aprikyan A.G., Bos C., Bonilla M.A., Boxer L.A., Kannourakis G., Zeidler C., Welte K., Benson K.F., Horwitz M.Blood 96:2317-2322(2000) Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease.Ancliff P.J., Gale R.E., Liesner R., Hann I.M., Linch D.C.Blood 98:2645-2650(2001) Paternal mosaicism proves the pathogenic nature of mutations in neutrophil elastase in severe congenital neutropenia.Ancliff P.J., Gale R.E., Watts M.J., Liesner R., Hann I.M., Strobel S., Linch D.C.Blood 100:707-709(2002) Mutations in the ELA2 gene correlate with more severe expression of neutropenia a study of 81 patients from the French Neutropenia Register.Bellanne-Chantelot C., Clauin S., Leblanc T., Cassinat B., Rodrigues-Lima F., Beaufils S., Vaury C., Barkaoui M., Fenneteau O., Maier-Redelsperger M., Chomienne C., Donadieu J.Blood 103:4119-4125(2004) Double de novo mutations of ELA2 in cyclic and severe congenital neutropenia.Salipante S.J., Benson K.F., Luty J., Hadavi V., Kariminejad R., Kariminejad M.H., Rezaei N., Horwitz M.S.Hum. Mutat. 28:874-881(2007) A novel mutation Ala57Val of the ELA2 gene in a Korean boy with severe congenital neutropenia.Lee S.T., Yoon H.S., Kim H.J., Lee J.H., Park J.H., Kim S.H., Seo J.J., Im H.J.Ann. Hematol. 88:593-595(2009) Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease a novel mutation in two unrelated British kindreds.Smith B.N., Ancliff P.J., Pizzey A., Khwaja A., Linch D.C., Gale R.E.Br. J. Haematol. 144:762-770(2009) Ela2 mutations and clinical manifestations in familial congenital neutropenia.Shiohara M., Shigemura T., Saito S., Tanaka M., Yanagisawa R., Sakashita K., Asada H., Ishii E., Koike K., Chin M., Kobayashi M., Koike K.J. Pediatr. Hematol. Oncol. 31:319-324(2009) Digenic mutations in severe congenital neutropenia.Germeshausen M., Zeidler C., Stuhrmann M., Lanciotti M., Ballmaier M., Welte K.Haematologica 95:1207-1210(2010) Pegfilgrastim in children with severe congenital neutropenia.Fioredda F., Calvillo M., Lanciotti M., Lanza T., Giunti L., Castagnola E., Lorenzi I., Tonelli R., Ghezzi P., Dufour C.Pediatr. Blood Cancer 54:465-467(2010) Severe congenital neutropenia in a multigenerational family with a novel neutrophil elastase (ELANE) mutation.van de Vosse E., Verhard E.M., Tool A.J., de Visser A.W., Kuijpers T.W., Hiemstra P.S., van Dissel J.T.Ann. Hematol. 90:151-158(2011) Four novel ELANE mutations in patients with congenital neutropenia.Kurnikova M., Maschan M., Dinova E., Shagina I., Finogenova N., Mamedova E., Polovtseva T., Shagin D., Shcherbina A.Pediatr. Blood Cancer 57:332-335(2011) The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia.Germeshausen M., Deerberg S., Peter Y., Reimer C., Kratz C.P., Ballmaier M.Hum. Mutat. 34:905-914(2013)

Small volumes of ELANE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS957715 is a recombinant protein targeting ELANE. Also known as Recombinant Human Neutrophil elastase (ELANE). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.