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3-Ketoacyl-Coa Thiolase (ACAA1) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS958322

MBS958322 is a recombinant protein targeting ACAA1. Also known as Recombinant Human 3-ketoacyl-CoA thiolase, peroxisomal. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
3-ketoacyl-CoA thiolase (ACAA1), Recombinant Protein
Full Product Name
Recombinant Human 3-ketoacyl-CoA thiolase, peroxisomal
Product Gene Name
Product Synonym Names
Acetyl-CoA acyltrans ferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase
Sequence
LSGAPQASAA DVVVVHGRRT AICRAGRGGF KDTTPDELLS AVMTAVLKDV NLRPEQLGDI CVGNVLQPGA GAIMARIAQF LSDIPETVPL STVNRQCSSG LQAVASIAGG IRNGSYDIGM ACGITSENVA ERFGISREKQ DTFALASQQK AARAQSKGCF QAEIVPVTTT VHDDKGTKRS ITVTQDEGIR PSTTMEGLAK LKPAFKKDGS TTAGLTVSDV DIFEINEAFA SQAAYCVEKL RLPPEKVNPL GGAVALGHPL GCTGARQVIT LLNELKRRGK RAYGVVSMCI GTGMGAAAVF EYPGN
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
47.9 kDa
Sequence Positions
27-331aa; Full Length of Isoform 2
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
604054
3D Structure
ModBase 3D Structure for P09110

Product Categories/Family for ACAA1 recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for ACAA1 recombinant protein

Nucleotide sequence of human peroxisomal 3-oxoacyl-CoA thiolase.Bout A., Teunissen Y., Hashimoto T., Benne R., Tager J.M.Nucleic Acids Res. 16:10369-10369(1988) Bout A.Complete cDNA sequence of human foetal liver peroxisomal 3-oxoacyl-CoA thiolase.Fairbairn L.J., Tanner M.J.A.Nucleic Acids Res. 17:3588-3588(1989) Characterization of the gene encoding human peroxisomal 3-oxoacyl-CoA thiolase (ACAA) . No large DNA rearrangement in a thiolase-deficient patient.Bout A., Franse M.M., Collins J., Blonden L., Tager J.M., Benne R.Biochim. Biophys. Acta 1090:43-51(1991) The DNA sequence, annotation and analysis of human chromosome 3.Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.Nature 440:1194-1198(2006) Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) Crystal structure of human peroxisomal acetyl-COA acyl transferase 1 (ACAA1) .Structural genomics consortium (SGC) Submitted (OCT-2006) to the PDB data bank

Other Notes

Small volumes of ACAA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACAA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
3-ketoacyl-CoA thiolase, peroxisomal isoform b
NCBI Official Synonym Full Names
acetyl-CoA acyltransferase 1
NCBI Official Symbol
ACAA1
NCBI Official Synonym Symbols
ACAA; THIO; PTHIO
UniProt Protein Name
3-ketoacyl-CoA thiolase, peroxisomal
UniProt Synonym Protein Names
Acetyl-CoA acyltransferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase
UniProt Gene Name
ACAA1
UniProt Synonym Gene Names
ACAA; PTHIO
UniProt Entry Name
THIK_HUMAN
NCBI Protein Information
3-ketoacyl-CoA thiolase, peroxisomal

NCBI Summary for ACAA1

This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

UniProt Comments for ACAA1

ACAA1: an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Protein type: Lipid Metabolism - fatty acid; Lipid Metabolism - unsaturated fatty acid biosynthesis; Acetyltransferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.3.1.16

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: intracellular membrane-bound organelle; membrane; peroxisomal matrix; peroxisome

Molecular Function: acetyl-CoA C-acyltransferase activity; palmitoyl-CoA oxidase activity; protein binding

Biological Process: bile acid metabolic process; cellular lipid metabolic process; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA oxidase; unsaturated fatty acid metabolic process; very-long-chain fatty acid metabolic process

Disease: D-bifunctional Protein Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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