Human PRIM1 Recombinant Protein – Cat# MBS965448

DNA Primase Small Subunit (PRIM1) Human Recombinant Protein

Name: PRIM1 Recombinant Protein; DNA Primase Small Subunit; Human; MyBioSource
Brand: MyBioSource
SKU: MBS965448
Price: 455 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS965448

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. More details

$455 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DNA primase small subunit (PRIM1), Recombinant Protein

Full Product Name

Recombinant Human DNA primase small subunit (PRIM1)

Product Gene Name

PRIM1 recombinant protein

Product Synonym Names

DNA primase small subunit; EC=2.7.7.-; DNA primase 49 kDa subunit; p49

Sequence

METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGVIKNYF QHREFSFTLK DDIYIRYQSF NNQSDLEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ AQEKELVFDI DMTDYDDVRR CCSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

65.9 kDa

Sequence Positions

1-420aa; Full Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

176635

3D Structure

ModBase 3D Structure for P49642

Species

Homo sapiens (Human)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PRIM1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRIM1 purchase

MBS965448 is a recombinant protein targeting PRIM1. Also known as Recombinant Human DNA primase small subunit (PRIM1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for PRIM1. It may not necessarily be applicable to this product.

NCBI GI #

4506051

NCBI GeneID

5557

NCBI Accession #

NP_000937.1

UniProt Primary Accession #

P49642

UniProt Related Accession #

P49642

GenBank Nucleotide #

NM_000946.2

NCBI Official Full Name

DNA primase small subunit

NCBI Official Synonym Full Names

primase, DNA, polypeptide 1 (49kDa)

NCBI Official Symbol

PRIM1

NCBI Official Synonym Symbols

p49

UniProt Protein Name

DNA primase small subunit

UniProt Synonym Protein Names

DNA primase 49 kDa subunit; p49

UniProt Gene Name

PRIM1

UniProt Synonym Gene Names

p49

UniProt Entry Name

PRI1_HUMAN

NCBI Protein Information

DNA primase small subunit; DNA primase 1; primase p49 subunit; DNA primase subunit 48; DNA primase 49 kDa subunit; primase polypeptide 1, 49kDa

NCBI Summary for PRIM1

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]

UniProt Comments for PRIM1

PRIM1: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. Heterodimer of a small subunit and a large subunit. Belongs to the eukaryotic-type primase small subunit family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transferase; EC 2.7.7.-; DNA replication

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; membrane

Molecular Function: DNA primase activity; metal ion binding

Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; telomere maintenance via recombination; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle

Research Articles on PRIM1

1. Findings indicate that tethering of DNA primase Prim1 to the replisome by DNA polymerase alpha (pol alpha) is critical for the normal action of DNA replication forks in eukaryotic cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRIM1 recombinant protein

Reference Product	PubMed Publications
PRIM2 recombinant protein	>3 publications with PRIM1 and PRIM2
MCM3 recombinant protein	>2 publications with PRIM1 and MCM3
MCM6 recombinant protein	>1 publications with PRIM1 and MCM6
RFC5 recombinant protein	>1 publications with PRIM1 and RFC5
PCNA recombinant protein	>1 publications with PRIM1 and PCNA
POLA1 recombinant protein	>1 publications with PRIM1 and POLA1
RFC4 recombinant protein	>1 publications with PRIM1 and RFC4

Pathways associated with PRIM1 recombinant protein

Products by Pathway	Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pre-Initiation Pathway antibodies	DNA Replication Pre-Initiation Pathway Diagram
DNA Polymerase Alpha / Primase Complex Pathway antibodies	DNA Polymerase Alpha / Primase Complex Pathway Diagram
DNA Polymerase Alpha / Primase Complex Pathway antibodies	DNA Polymerase Alpha / Primase Complex Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram

Diseases associated with PRIM1 recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>1 publications with PRIM1 and Disease Models, Animal
Cognition Disorders Antibodies	>1 publications with PRIM1 and Cognition Disorders
Nervous System Diseases Antibodies	>1 publications with PRIM1 and Nervous System Diseases
Cardiovascular Diseases Antibodies	>1 publications with PRIM1 and Cardiovascular Diseases
Heart Diseases Antibodies	>1 publications with PRIM1 and Heart Diseases

Organs/Tissues associated with PRIM1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>2 publications with PRIM1 and Embryonic Tissue
Bone Antibodies	>1 publications with PRIM1 and Bone
Eye Antibodies	>1 publications with PRIM1 and Eye
Lung Antibodies	>1 publications with PRIM1 and Lung
Brain Antibodies	>1 publications with PRIM1 and Brain