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Vitamin B12-Binding Protein (SerAS13_0720) E Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS965457
PROMOTIONAL PRICING: 10% OFF web price. Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed

Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vitamin B12-binding protein (SerAS13_0720), Recombinant Protein
Full Product Name
Recombinant E Coli Vitamin B12-binding protein
Product Synonym Gene Name
btuF; yadT; b0158; JW0154; VB12
Sequence
PRVITLSPAN TELAFAAGIT PVGVSSYSDY PPQAQKIEQV STWQGMNLER IVALKPDLVI AWRGGNAERQ VDQLASLGIK VMWVDATSIE QIANALRQLA PWSPQPDKAE QAAQSLLDQY AQLKAQYADK PKKRVFLQFG INPPFTSGKE SIQNQVLEVC GGENIFKDSR VPWPQVSREQ VLARSPQAIV ITGGPDQIPK IKQYWGEQLK IPVIPLTSDW FERASPRIIL AAQQLCNALS QVD
Host
E Coli
Form/Format
Liquid containing glycerol
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
30.9 kDa
Sequence Positions
24-266aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P37028
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for SerAS13_0720 recombinant protein

Systematic sequencing of the Escherichia coli genome analysis of the 2.4-4.1 min (110,917-193,643 bp) region.Fujita N., Mori H., Yura T., Ishihama A.Nucleic Acids Res. 22:1637-1639(1994) Sequence of minutes 4-25 of Escherichia coli.Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli.Cadieux N., Bradbeer C., Reeger-Schneider E., Koester W., Mohanty A.K., Wiener M.C., Kadner R.J.J. Bacteriol. 184:706-717(2002) The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter.Borths E.L., Locher K.P., Lee A.T., Rees D.C.Proc. Natl. Acad. Sci. U.S.A. 99:16642-16647(2002) Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding.Karpowich N.K., Huang H.H., Smith P.C., Hunt J.F.J. Biol. Chem. 278:8429-8434(2003)

Other Notes

Small volumes of SerAS13_0720 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SerAS13_0720 purchase

MBS965457 is a recombinant protein targeting SerAS13_0720. Also known as Recombinant E Coli Vitamin B12-binding protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SerAS13_0720. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
vitamin B12 ABC transporter periplasmic binding protein
NCBI Official Symbol
btuF
NCBI Official Synonym Symbols
ECK0157; JW0154; yadT
UniProt Protein Name
Vitamin B12-binding protein
UniProt Gene Name
btuF
UniProt Synonym Gene Names
yadT
UniProt Entry Name
BTUF_ECOLI
NCBI Protein Information
vitamin B12 ABC transporter periplasmic binding protein

NCBI Summary for SerAS13_0720

Uncleaved precursor N-terminus was also sequenced, confirming this start site of translation. about 80% of the precursors had the initial Met cleaved. [More information is available at EcoGene: EG12334]. BtuF is the periplasmic vitamin B12 binding protein that delivers cobalamin to the ABC transporter BtuCD. [More information is available at EcoCyc: EG12334].
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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