Heparan Sulfate N-Deacetylase/N-Sulfotransferase 1 (NDST1) Human Bifunctional Recombinant Protein

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1.Dixon J., Loftus S.K., Gladwin A.J., Scambler P.J., Wasmuth J.J., Dixon M.J.Genomics 26:239-244(1995) Localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase to the trans-Golgi network.Humphries D.E., Sullivan B.M., Aleixo M.D., Stow J.L.Biochem. J. 325:351-357(1997) Labell T.L., Milewicz D.J., Bonadio J., Edelhoff S., Disteche C.M., Byers P.H. A role of Lys614 in the sulfotransferase activity of human heparan sulfate N-deacetylase/N-sulfotransferase.Sueyoshi T., Kakuta Y., Pedersen L.C., Wall F.E., Pedersen L.G., Negishi M.FEBS Lett. 433:211-214(1998) Overexpression of different isoforms of glucosaminyl N-deacetylase/N-sulfotransferase results in distinct heparan sulfate N-sulfation patterns.Pikas D.S., Eriksson I., Kjellen L.Biochemistry 39:4552-4558(2000) Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST) novel characteristics of NDST-1 and -2.van den Born J., Pikas D.S., Pisa B.J., Eriksson I., Kjellen L., Berden J.H.M.Glycobiology 13:1-10(2003) Crystal structure of the sulfotransferase domain of human heparan sulfate N-deacetylase/N-sulfotransferase 1.Kakuta Y., Sueyoshi T., Negishi M., Pedersen L.C.J. Biol. Chem. 274:10673-10676(1999) NDST1 missense mutations in autosomal recessive intellectual disability.Reuter M.S., Musante L., Hu H., Diederich S., Sticht H., Ekici A.B., Uebe S., Wienker T.F., Bartsch O., Zechner U., Oppitz C., Keleman K., Jamra R.A., Najmabadi H., Schweiger S., Reis A., Kahrizi K.Am. J. Med. Genet. A 164A:2753-2763(2014)

Small volumes of NDST1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS966196 is a recombinant protein targeting NDST1. Also known as Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.