Mouse LYPLA1 Recombinant Protein – Cat# MBS966360

Acyl-Protein Thioesterase 1 (LYPLA1) Mouse Recombinant Protein

Name: LYPLA1 Recombinant Protein; Acyl-Protein Thioesterase 1; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS966360
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS966360

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Acyl-protein thioesterase 1 (Lypla1), Recombinant Protein

Full Product Name

Recombinant Mouse Acyl-protein thioesterase 1

Product Gene Name

Lypla1 recombinant protein

Product Synonym Gene Name

Apt1; Pla1a

Product Synonym Names

Lysophospholipase 1; Lysophospholipase I; LPL-I; Lyso; PLA I

Sequence

MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

51.7 kDa

Sequence Positions

1-230. Full-Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for P97823

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for Lypla1 recombinant protein

Cloning, expression, and catalytic mechanism of murine lysophospholipase I.Wang A., Deems R.A., Dennis E.A.J. Biol. Chem. 272:12723-12729(1997) Lubec G., Yang J.W., Zigmond M.Submitted (JUL-2007) to UniProtKB Regiospecificity and catalytic triad of lysophospholipase I.Wang A., Loo R., Chen Z., Dennis E.A.J. Biol. Chem. 272:22030-22036(1997) Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013)

Other Notes

Small volumes of Lypla1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Lypla1 purchase

MBS966360 is a recombinant protein targeting Lypla1. Also known as Recombinant Mouse Acyl-protein thioesterase 1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Lypla1. It may not necessarily be applicable to this product.

NCBI GI #

6678760

NCBI GeneID

18777

NCBI Accession #

NP_032892.1

UniProt Primary Accession #

P97823

UniProt Secondary Accession #

Q3TJZ0; Q7TPX1; Q8BWM6

UniProt Related Accession #

P97823

GenBank Nucleotide #

NM_008866.2

NCBI Official Full Name

acyl-protein thioesterase 1

NCBI Official Synonym Full Names

lysophospholipase 1

NCBI Official Symbol

Lypla1

NCBI Official Synonym Symbols

Pla1a

UniProt Protein Name

Acyl-protein thioesterase 1

UniProt Synonym Protein Names

Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I

Protein Family

Acyl-protein thioesterase

UniProt Gene Name

Lypla1

UniProt Synonym Gene Names

Apt1; Pla1a; APT-1; LPL-I; LysoPLA I

UniProt Entry Name

LYPA1_MOUSE

NCBI Protein Information

acyl-protein thioesterase 1

UniProt Comments for Lypla1

LYPLA1: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity and also low lysophospholipase activity. Belongs to the AB hydrolase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Phospholipase; EC 3.1.2.-

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function: acyl-CoA hydrolase activity; CoA hydrolase activity; hydrolase activity; lipase activity; lysophospholipase activity; palmitoyl-(protein) hydrolase activity

Biological Process: fatty acid metabolic process; lipid metabolic process; negative regulation of Golgi to plasma membrane protein transport; protein depalmitoylation

Research Articles on Lypla1

1. Data indicate that thioesterases APT1/APT2 depalmitoylate nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) and zDHHC17 is the strongest candidate palmitoyltransferase for NMNAT2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Lypla1 recombinant protein

Reference Product	PubMed Publications
Lpcat2 recombinant protein	>1 publications with Lypla1 and Lpcat2

Pathways associated with Lypla1 recombinant protein

Products by Pathway	Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nitric Oxide Pathway antibodies	Metabolism Of Nitric Oxide Pathway Diagram
ENOS Activation Pathway antibodies	ENOS Activation Pathway Diagram
ENOS Activation And Regulation Pathway antibodies	ENOS Activation And Regulation Pathway Diagram

Organs/Tissues associated with Lypla1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>6 publications with Lypla1 and Blood
Brain Antibodies	>6 publications with Lypla1 and Brain
Kidney Antibodies	>3 publications with Lypla1 and Kidney
Intestine Antibodies	>1 publications with Lypla1 and Intestine
Bladder Antibodies	>1 publications with Lypla1 and Bladder
Skin Antibodies	>1 publications with Lypla1 and Skin
Thymus Antibodies	>1 publications with Lypla1 and Thymus