Non-Secretory Ribonuclease (RNASE2) Human Recombinant Protein

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chotactic for dendritic cells. Possesses a wide variety of biological activities.

Sequence of human eosinophil-derived neurotoxin cDNA identity of deduced amino acid sequence with human nonsecretory ribonucleases.Hamann K.J., Barker R.L., Loegering D.A., Pease L.R., Gleich G.J.Gene 83:161-167(1989) Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases.Barker R.L., Loegering D.A., Ten R.M., Hamann K.J., Pease L.R., Gleich G.J.J. Immunol. 143:952-955(1989) Molecular cloning of the human eosinophil-derived neurotoxin a member of the ribonuclease gene family.Rosenberg H.F., Tenen D.G., Ackerman S.J.Proc. Natl. Acad. Sci. U.S.A. 86:4460-4464(1989) Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes evidence for intronless coding sequences in the ribonuclease gene superfamily.Hamann K.J., Ten R.M., Loegering D.A., Jenkins R.B., Heise M.T., Schad C.R., Pease L.R., Gleich G.J., Barker R.L.Genomics 7:535-546(1990) Sequence variation at two eosinophil-associated ribonuclease loci in humans.Zhang J., Rosenberg H.F.Genetics 156:1949-1958(2000) Simonsen C.C., Kennedy J., Comstock L., Ashton N., McGrogan M. Amino acid sequence of the nonsecretory ribonuclease of human urine.Beintema J.J., Hofsteenge J., Iwama M., Morita T., Ohgi K., Irie M., Sugiyama R.H., Schieven G.L., Dekker C.A., Glitz D.G.Biochemistry 27:4530-4538(1988) Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein homology with ribonuclease.Gleich G.J., Loegering D.A., Bell M.P., Checkel J.L., Ackerman S.J., McKean D.J.Proc. Natl. Acad. Sci. U.S.A. 83:3146-3150(1986) Purification and properties of bovine kidney ribonucleases.Niwata Y., Ohgi K., Sanda A., Takizawa Y., Irie M.J. Biochem. 97:923-934(1985) Purification and characterization of a ribonuclease from human liver.Sorrentino S., Tucker G.K., Glitz D.G.J. Biol. Chem. 263:16125-16131(1988) Characterization of a unique nonsecretory ribonuclease from urine of pregnant women.Sakakibara R., Hashida K., Kitahara T., Ishiguro M.J. Biochem. 111:325-330(1992) Characterisation of UGP and its relationship with beta-core fragment.Kardana A., Bagshawe K.D., Coles B., Read D., Taylor M.Br. J. Cancer 67:686-692(1993) New type of linkage between a carbohydrate and a protein C-glycosylation of a specific tryptophan residue in human RNase Us.Hofsteenge J., Mueller D.R., de Beer T., Loeffler A., Richter W.J., Vliegenthart J.F.G.Biochemistry 33:13524-13530(1994) The hexopyranosyl residue that is C-glycosidically linked to the side chain of tryptophan-7 in human RNase Us is alpha-mannopyranose.de Beer T., Vliegenthart J.F.G., Loeffler A., Hofsteenge J.Biochemistry 34:11785-11789(1995) Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp.Krieg J., Hartmann S., Vicentini A., Glasner W., Hess D., Hofsteenge J.Mol. Biol. Cell 9:301-309(1998) Mutational analysis of the complex of human RNase inhibitor and human eosinophil-derived neurotoxin (RNase 2) .Teufel D.P., Kao R.Y., Acharya K.R., Shapiro R.Biochemistry 42:1451-1459(2003) Eosinophil-derived neurotoxin (EDN) , an antimicrobial protein with chemotactic activities for dendritic cells.Yang D., Rosenberg H.F., Chen Q., Dyer K.D., Kurosaka K., Oppenheim J.J.Blood 102:3396-3403(2003) Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase.Ulrich M., Petre A., Youhnovski N., Proemm F., Schirle M., Schumm M., Pero R.S., Doyle A., Checkel J., Kita H., Thiyagarajan N., Acharya K.R., Schmid-Grendelmeier P., Simon H.-U., Schwarz H., Tsutsui M., Shimokawa H., Bellon G., Lee J.J., Przybylski M., Doering G.J. Biol. Chem. 283:28629-28640(2008) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) X-ray crystallographic structure of recombinant eosinophil-derived neurotoxin at 1.83-A resolution.Mosimann S.C., Newton D.L., Youle R.J., James M.N.G.J. Mol. Biol. 260:540-552(1996) Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN) . High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors.Leonidas D.D., Boix E., Prill R., Suzuki M., Turton R., Minson K., Swaminathan G.J., Youle R.J., Acharya K.R.J. Biol. Chem. 276:15009-15017(2001) Molecular recognition of human eosinophil-derived neurotoxin (RNase 2) by placental ribonuclease inhibitor.Iyer S., Holloway D.E., Kumar K., Shapiro R., Acharya K.R.J. Mol. Biol. 347:637-655(2005) Crystal structures of eosinophil-derived neurotoxin (EDN) in complex with the inhibitors 5'-ATP, Ap3A, Ap4A, and Ap5A.Baker M.D., Holloway D.E., Swaminathan G.J., Acharya K.R.Biochemistry 45:416-426(2006)

Small volumes of RNASE2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS967723 is a recombinant protein targeting RNASE2. Also known as Recombinant Human Non-secretory ribonuclease. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.