Human ACTA1 Recombinant Protein – Cat# MBS968277

Actin, Alpha Skeletal Muscle (ACTA1) Human Recombinant Protein

Name: ACTA1 Recombinant Protein; Actin, Alpha Skeletal Muscle; Human; MyBioSource
Brand: MyBioSource
SKU: MBS968277
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS968277

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. More details

$335 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Actin, alpha skeletal muscle (ACTA1), Recombinant Protein

Full Product Name

Recombinant Human Actin, alpha skeletal muscle (ACTA1)

Product Gene Name

ACTA1 recombinant protein

Product Synonym Names

Alpha-actin-1

Sequence

DEDETTALVC DNGSGLVKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIITNWD DMEKIWHHTF YNELRVAPEE HPTLLTEAPL NPKANREKMT QIMFETFNVP AMYVAIQAVL SLYASGRTTG IVLDSGDGVT HNVPIYEGYA LPHAIMRLDL AGRDLTDYLM KILTERGYSF VTTAEREIVR DIKEKLCYVA LDFENEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPET LFQPSFIGME SAGIHETTYN SIMKCDIDIR KDLYANNVMS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWITKQ EYDEAGPSIV HRKCF

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

45.8 kDa

Sequence Positions

3-377aa; Full Length of Mature Protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

102610

3D Structure

ModBase 3D Structure for P68133

Product Categories/Family for ACTA1 recombinant protein

Signal Transduction

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for ACTA1 recombinant protein

Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.Hanauer A., Levin M., Heilig R., Daegelen D., Kahn A., Mandel J.-L.Nucleic Acids Res. 11:3503-3516(1983) Nucleotide sequence and expression of the human skeletal alpha-actin gene evolution of functional regulatory domains.Taylor A., Erba H.P., Muscat G.E.O., Kedes L.Genomics 3:323-336(1988) Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy.Nowak K.J., Wattanasirichaigoon D., Goebel H.H., Wilce M., Pelin K., Donner K., Jacob R.L., Hubner C., Oexle K., Anderson J.R., Verity C.M., North K.N.Nat. Genet. 23:208-212(1999)

Other Notes

Small volumes of ACTA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA1 purchase

MBS968277 is a recombinant protein targeting ACTA1. Also known as Recombinant Human Actin, alpha skeletal muscle (ACTA1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.

NCBI GI #

4501881

NCBI GeneID

NCBI Accession #

NP_001091.1

UniProt Primary Accession #

P68133

UniProt Secondary Accession #

P02568; P99020; Q5T8M9

UniProt Related Accession #

P68133

GenBank Nucleotide #

NM_001100.3

NCBI Official Full Name

actin, alpha skeletal muscle

NCBI Official Synonym Full Names

actin, alpha 1, skeletal muscle

NCBI Official Symbol

ACTA1

NCBI Official Synonym Symbols

ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM

UniProt Protein Name

Actin, alpha skeletal muscle

UniProt Synonym Protein Names

Alpha-actin-1

UniProt Gene Name

ACTA1

UniProt Synonym Gene Names

ACTA

UniProt Entry Name

ACTS_HUMAN

NCBI Protein Information

actin, alpha skeletal muscle

NCBI Summary for ACTA1

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTA1

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Contractile; Motility/polarity/chemotaxis; Cell development/differentiation; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: actin cytoskeleton; actin filament; cytosol; extracellular space; filopodium; lamellipodium; sarcomere; stress fiber; striated muscle thin filament

Molecular Function: ADP binding; ATP binding; myosin binding; protein binding; structural constituent of cytoskeleton

Biological Process: cell growth; muscle contraction; muscle filament sliding; muscle thin filament assembly; response to extracellular stimulus; response to lithium ion; response to mechanical stimulus; response to steroid hormone stimulus; skeletal muscle fiber adaptation; skeletal muscle fiber development

Disease: Myopathy, Congenital, With Fiber-type Disproportion; Nemaline Myopathy 3

Research Articles on ACTA1

1. Upon actin engagement, the N-terminal "strap" and helix 1 are displaced from the vinculin tail helical bundle to mediate actin bundling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACTA1 recombinant protein

Reference Product	PubMed Publications
GSN recombinant protein	>2 publications with ACTA1 and GSN
DMD recombinant protein	>1 publications with ACTA1 and DMD

Pathways associated with ACTA1 recombinant protein

Products by Pathway	Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Hypothetical Network For Drug Addiction Pathway antibodies	Hypothetical Network For Drug Addiction Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram
RhoA Signaling Pathway antibodies	RhoA Signaling Pathway Diagram
Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies	Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram
Striated Muscle Contraction Pathway antibodies	Striated Muscle Contraction Pathway Diagram

Diseases associated with ACTA1 recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>100 publications with ACTA1 and Nervous System Diseases
MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION Antibodies	>78 publications with ACTA1 and MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION
Heart Diseases Antibodies	>22 publications with ACTA1 and Heart Diseases
Hypertrophy Antibodies	>21 publications with ACTA1 and Hypertrophy
Cardiovascular Diseases Antibodies	>20 publications with ACTA1 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>14 publications with ACTA1 and Disease Models, Animal
Fibrosis Antibodies	>8 publications with ACTA1 and Fibrosis
Brain Diseases Antibodies	>6 publications with ACTA1 and Brain Diseases
Inflammation Antibodies	>5 publications with ACTA1 and Inflammation
Hypertension Antibodies	>2 publications with ACTA1 and Hypertension

Organs/Tissues associated with ACTA1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>142 publications with ACTA1 and Muscle
Heart Antibodies	>36 publications with ACTA1 and Heart
Brain Antibodies	>14 publications with ACTA1 and Brain
Embryonic Tissue Antibodies	>8 publications with ACTA1 and Embryonic Tissue
Bone Antibodies	>7 publications with ACTA1 and Bone
Nerve Antibodies	>6 publications with ACTA1 and Nerve
Lung Antibodies	>5 publications with ACTA1 and Lung
Vascular Antibodies	>3 publications with ACTA1 and Vascular
Liver Antibodies	>3 publications with ACTA1 and Liver
Adipose Tissue Antibodies	>2 publications with ACTA1 and Adipose Tissue