E3 SUMO-Protein Ligase PIAS3 (PIAS3) Antibody – Rabbit Polyclonal

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: SUMO conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; dendrite; nuclear speck; nucleoplasm; synapse

Molecular Function: enzyme binding; ligase activity; potassium channel regulator activity; protein binding; protein C-terminus binding; protein N-terminus binding; SUMO ligase activity; zinc ion binding

Biological Process: cellular protein metabolic process; negative regulation of protein sumoylation; positive regulation of membrane potential; positive regulation of protein sumoylation; post-translational protein modification; protein sumoylation; regulation of transcription, DNA-dependent; response to hormone stimulus; transcription, DNA-dependent