Prekallikrein (PK) ELISA Kit for Mouse

Name: Mouse PK ELISA Kit; Prekallikrein; MyBioSource
Brand: MyBioSource
SKU: MBS097954
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS097954

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of PK (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates,… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Prekallikrein (PK), ELISA Kit

Full Product Name

Mouse Prekallikrein ELISA Kit

Product Gene Name

PK elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Detection Range

6.25 umol/L - 200 umol/L.

Sensitivity

1.0 umol/L.

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

71,370 Da

OMIM

229000

3D Structure

ModBase 3D Structure for P03952

Other Notes

Small volumes of PK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PK purchase

MBS097954 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prekallikrein (PK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PK. The ELISA analytical biochemical technique of the MBS097954 kit is based on PK antibody-PK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PK. It may not necessarily be applicable to this product.

NCBI GI #

78191798

NCBI GeneID

3818

NCBI Accession #

NP_000883.2

UniProt Primary Accession #

P03952

UniProt Secondary Accession #

Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9

UniProt Related Accession #

P03952

GenBank Nucleotide #

NM_000892.3

NCBI Official Full Name

plasma kallikrein preproprotein

NCBI Official Synonym Full Names

kallikrein B, plasma (Fletcher factor) 1

NCBI Official Symbol

KLKB1

NCBI Official Synonym Symbols

PPK; KLK3

UniProt Protein Name

Plasma kallikrein

UniProt Synonym Protein Names

Fletcher factor; Kininogenin; Plasma prekallikrein

Protein Family

Pyridoxal kinase

UniProt Gene Name

KLKB1

UniProt Synonym Gene Names

KLK3

UniProt Entry Name

KLKB1_HUMAN

NCBI Protein Information

plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain

NCBI Summary for PK

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

UniProt Comments for PK

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; fibrinolysis; positive regulation of fibrinolysis; zymogen activation; blood coagulation; proteolysis; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency

Research Articles on PK

1. The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PK elisa kit

Reference Product	PubMed Publications
F12 elisa kit	>3 publications with PK and F12
KNG1 elisa kit	>1 publications with PK and KNG1

Pathways associated with PK elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Blood Clotting Cascade Pathway antibodies	Blood Clotting Cascade Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Intrinsic Pathway antibodies	Intrinsic Pathway Diagram

Diseases associated with PK elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>8 publications with PK and Cardiovascular Diseases
Hypertension Antibodies	>4 publications with PK and Hypertension
Neoplasms Antibodies	>4 publications with PK and Neoplasms
Kidney Diseases Antibodies	>2 publications with PK and Kidney Diseases
Prostatic Diseases Antibodies	>1 publications with PK and Prostatic Diseases
Brain Diseases Antibodies	>1 publications with PK and Brain Diseases
Mental Disorders Antibodies	>1 publications with PK and Mental Disorders
Inflammation Antibodies	>1 publications with PK and Inflammation
Liver Diseases Antibodies	>1 publications with PK and Liver Diseases
Death Antibodies	>1 publications with PK and Death

Organs/Tissues associated with PK elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with PK and Kidney
Heart Antibodies	>2 publications with PK and Heart
Brain Antibodies	>2 publications with PK and Brain
Liver Antibodies	>2 publications with PK and Liver
Connective Tissue Antibodies	>1 publications with PK and Connective Tissue
Mammary Gland Antibodies	>1 publications with PK and Mammary Gland