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Actin, Cytoplasmic 1 (ACTB) ELISA Kit for Duck

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9354687

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of ACTB (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Actin, Cytoplasmic 1 (ACTB), ELISA Kit
Full Product Name
Duck Actin, Cytoplasmic 1 (ACTB) ELISA Kit
Product Gene Name
ACTB elisa kit
Species Reactivity
Duck
Assay Type
Quantitative Sandwich
Detection Range
3.12ng/ml-100ng/ml
Sensitivity
1.0ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
41,737 Da
OMIM
102630

Other Notes

Small volumes of ACTB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS9354687 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actin, Cytoplasmic 1 (ACTB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTB. The ELISA analytical biochemical technique of the MBS9354687 kit is based on ACTB antibody-ACTB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
4501885
NCBI GeneID
60
NCBI Accession #
NP_001092.1
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
GenBank Nucleotide #
NM_001101.3
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTB

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; membrane; NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; plasma membrane; protein complex; ribonucleoprotein complex

Molecular Function: ATP binding; identical protein binding; kinesin binding; nitric-oxide synthase binding; nucleosomal DNA binding; protein binding; structural constituent of cytoskeleton; Tat protein binding

Biological Process: 'de novo' posttranslational protein folding; ATP-dependent chromatin remodeling; axon guidance; blood coagulation; cell motility; cellular protein metabolic process; ephrin receptor signaling pathway; establishment and/or maintenance of chromatin architecture; gene expression; innate immune response; intercellular junction assembly and maintenance; positive regulation of gene expression, epigenetic; protein folding; regulation of gene expression, epigenetic; retinal homeostasis; small GTPase mediated signal transduction; substantia nigra development; vascular endothelial growth factor receptor signaling pathway

Disease: Baraitser-winter Syndrome 1; Dystonia, Juvenile-onset
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Research Articles on ACTB

1. Data show that tripartite motif-containing 28 protein (TRIM28) and beta-actin were up-regulated in the glioblastoma multiforme (GBM) stem-like cells compared to the controls.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACTB elisa kit

 Reference Product  PubMed Publications
 UBC elisa kit  >42 publications with ACTB and UBC
 POLR2A elisa kit  >15 publications with ACTB and POLR2A
 CFL1 elisa kit  >2 publications with ACTB and CFL1
 PFN1 elisa kit  >1 publications with ACTB and PFN1

Pathways associated with ACTB elisa kit

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies  B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram

Diseases associated with ACTB elisa kit

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >55 publications with ACTB and Carcinoma
 Liver Diseases Antibodies  >24 publications with ACTB and Liver Diseases
 Disease Models, Animal Antibodies  >23 publications with ACTB and Disease Models, Animal
 Lung Diseases Antibodies  >20 publications with ACTB and Lung Diseases
 Inflammation Antibodies  >18 publications with ACTB and Inflammation
 Necrosis Antibodies  >18 publications with ACTB and Necrosis
 Kidney Diseases Antibodies  >17 publications with ACTB and Kidney Diseases
 Lung Neoplasms Antibodies  >15 publications with ACTB and Lung Neoplasms
 Liver Neoplasms Antibodies  >13 publications with ACTB and Liver Neoplasms
 Heart Diseases Antibodies  >12 publications with ACTB and Heart Diseases

Organs/Tissues associated with ACTB elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >91 publications with ACTB and Blood
 Brain Antibodies  >63 publications with ACTB and Brain
 Muscle Antibodies  >59 publications with ACTB and Muscle
 Liver Antibodies  >59 publications with ACTB and Liver
 Kidney Antibodies  >39 publications with ACTB and Kidney
 Bone Antibodies  >34 publications with ACTB and Bone
 Lung Antibodies  >33 publications with ACTB and Lung
 Heart Antibodies  >30 publications with ACTB and Heart
 Connective Tissue Antibodies  >24 publications with ACTB and Connective Tissue
 Embryonic Tissue Antibodies  >21 publications with ACTB and Embryonic Tissue
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