Adpsensor Universal Kinase Assay Kit for General

Background/Introduction: Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn't interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (<0.05 uM ADP) with broad ATP tolerance (up to 1 mM). The kit can detect as low as 1 ng of active Kinase.

Small volumes of ADPsensor Universal Kinase assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS846583 is an assay kit targeting ADPsensor Universal Kinase. Also known as ADPsensor Universal Kinase Activity Assay Kit. Reactive in General.