BAP1 ELISA Kits

Initially identified as a BRCA1-binding protein, BAP1 is a deubiquitinating enzyme with C-terminal active hydrolase domain (UCH) and N-terminal nuclear localization signals (NLS1, NLS2). Its tumor suppressor functions have been recently described and it has been found that BAP1 plays a role in cycle-cell progression, DNA ionizing radiation breaks repair and gene expression regulation. Recently, variants of the BRCA1-associated protein 1 (BAP1) gene resulting in nuclear protein loss were reported in hereditary and sporadic mesothelioma. Using immunohistochemistry, a study evaluated the utility of BAP1 expression in the differential diagnosis between mesothelioma and other mesothelial proliferations on a large series of biopsies. BAP1 stain also showed utility in the differential of mesothelioma from most common pleural and peritoneal mimickers, such as lung and ovary carcinomas, with specificity and sensitivity of 99/70% and 100/70%, respectively. The results show that BAP1 protein is frequently lost in mesothelioma, especially of epithelioid/biphasic subtype and is commonly associated with homozygous BAP1 deletion. BAP1 immunostain represents an excellent biomarker with an unprecedented specificity (100%) in the distinction between benign and malignant mesothelial proliferations. Finding BAP1 loss in mesothelial cells should prompt to immediately reevaluate the patient; moreover, it might be useful in mapping tumor extent and planning surgical resection.

Also, a research identified a novel cancer syndrome related to germline BRCA1-associated protein 1 (BAP1) loss-of-function mutations and inherited with dominant autosomal transmission. Family members bearing BAP1 germline mutations show increased susceptibility to develop a variety of neoplasms, including uveal melanoma, cutaneous melanoma, atypical Spitz tumor, clear cell renal cancer, basal cell carcinoma, and mesothelioma, the latter occurring independently from occupational or environmental asbestos exposure.