• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Blocker Blocking Buffer Accessory Reagent – Supplied at 1X.

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258397

Background: General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes… More details

$190 / 100 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Blocker Blocking Buffer, Accessory Reagent
Full Product Name
General Blocker Blocking Buffer
Form/Format
Supplied at 1X.
Preparation and Storage
Store at 2-8 degree C.
pH
7.4 at 1X
Contains
Mammalian Proteins.
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.

Other Notes

Small volumes of Blocker Blocking Buffer accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Blocker Blocking Buffer purchase

MBS258397 is an accessory reagent targeting Blocker Blocking Buffer. Also known as General Blocker Blocking Buffer.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000