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CD154 (CD40LG) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638227

CD40 Ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 34 39kD type II More details

$1015 / 0.025 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD154 (CD40LG), Active Protein
Full Product Name
CD154, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5)
Product Gene Name
CD40LG active protein
Form/Format
Supplied as a lyophilized powder in PBS, EDTA. Reconstitute with 100ug/ml in PBS.
Purity/Purification
Highly Purified
~95% (SDS-PAGE)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
29,274 Da
Chromosome Location
Chromosome: X; NC_000023.10 (135730281..135742549). Location: Xq26
OMIM
300386
3D Structure
ModBase 3D Structure for P29965
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is typically 0.2-1.2ng/ml in the presence of 20ng/ml of recombinant human IL-4 and a cross-linking antibody mouse anti-HA.
Endotoxin Level
<1EU/ug (LAL method)

Product Categories/Family for CD40LG active protein

Growth Factors; Cytokines; Growth Factors-TRAP

Other Notes

Small volumes of CD40LG active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD40LG purchase

MBS638227 is an active protein targeting CD40LG. Also known as CD154, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5).

Additional Details

NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.

NCBI GI #
4557433
NCBI GeneID
959
NCBI Accession #
NP_000065.1
UniProt Primary Accession #
P29965
UniProt Related Accession #
P29965
GenBank Nucleotide #
NM_000074.2
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Protein Family
CD40 ligand
UniProt Gene Name
CD40LG
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP
UniProt Entry Name
CD40L_HUMAN
NCBI Protein Information
CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5

NCBI Summary for CD40LG

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

UniProt Comments for CD40LG

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1
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Research Articles on CD40LG

1. A major part of central and effector memory CD8(+) T cells expresses CD40L, one key molecule for CD4(+) T-cell-mediated help.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD40LG active protein

 Reference Product  PubMed Publications
 CD40 active protein  >23 publications with CD40LG and CD40
 ICAM1 active protein  >3 publications with CD40LG and ICAM1
 CD86 active protein  >3 publications with CD40LG and CD86
 TRAF1 active protein  >2 publications with CD40LG and TRAF1
 RELA active protein  >1 publications with CD40LG and RELA

Pathways associated with CD40LG active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram

Diseases associated with CD40LG active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with CD40LG and Inflammation
 Neoplasms Antibodies  >7 publications with CD40LG and Neoplasms
 Necrosis Antibodies  >6 publications with CD40LG and Necrosis
 Myocardial Infarction Antibodies  >3 publications with CD40LG and Myocardial Infarction
 IMMUNODEFICIENCY WITH HYPER-IgM, TYPE 1 Antibodies  >3 publications with CD40LG and IMMUNODEFICIENCY WITH HYPER-IgM, TYPE 1
 Myocardial Ischemia Antibodies  >3 publications with CD40LG and Myocardial Ischemia
 Cardiovascular Diseases Antibodies  >3 publications with CD40LG and Cardiovascular Diseases
 Heart Diseases Antibodies  >3 publications with CD40LG and Heart Diseases
 Kidney Diseases Antibodies  >2 publications with CD40LG and Kidney Diseases
 Hypersensitivity Antibodies  >2 publications with CD40LG and Hypersensitivity

Organs/Tissues associated with CD40LG active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >28 publications with CD40LG and Blood
 Mixed Antibodies  >1 publications with CD40LG and Mixed
 Thymus Antibodies  >1 publications with CD40LG and Thymus
 Brain Antibodies  >1 publications with CD40LG and Brain
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