Reverse Transcriptase Reagent – Cat# MBS9141650

Reverse Transcriptase Reagent – 200,000 U/ml

Name: Reverse Transcriptase Reagent; 200,000 U/ml; MyBioSource
Brand: MyBioSource
SKU: MBS9141650
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9141650

Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48… More details

$245 / 4000 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Reverse Transcriptase, Reagent

Full Product Name

Reverse Transcriptase

Host

E Coli

Form/Format

20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 at 25 degree C

Concentration

200,000 U/ml (lot specific)

Preparation and Storage

Store at -20 degree C.

Source Note

The gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H-) is expressed in E Coli and purified to near homogeneity.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 mul in 10 minutes at 37 degree C using poly(rA)ooligo(dT)18 as template.

Reaction Conditions

1X First-Strand Buffer, 10 mM DTT, 200 units ABScript II Reverse Transcriptase, supplemented with 0.5 mM dNTPs (not included) and 5 uM dT23VN (not included). Incubate at 42 degree C for 50 minutes.

Reaction Buffer or Mix

1X First-Strand Buffer Reaction Buffer:
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, pH 8.3 at 25 degree C

Heat Inactivation

65 degree C for 20 min

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Applications Tested/Suitable for Reverse Transcriptase reagent

cDNA Synthesis, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR

Other Notes

Small volumes of Reverse Transcriptase reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Reverse Transcriptase purchase

MBS9141650 is a reagent targeting Reverse Transcriptase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Reverse Transcriptase. It may not necessarily be applicable to this product.

Protein Family

Reverse transcriptase-like protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.