Jurkat cells were treated with 1?M Camptothecin and detected by this kit:

Annexin V Kit

Name: Kit; Annexin V; MyBioSource
Brand: MyBioSource
SKU: MBS2557014
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2557014

Background: Elabscience Annexin V V-FITC/PI Apoptosis Kit is developed to detect the apoptosis of suspension cells and adherent cells. Annexin V is a member of the annexin family, which binds to phosphatidylser ine (PS) in a calcium calcium-dependent manner.… More details

$220 / 20 Assays +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Annexin V, Kit

Full Product Name

Annexin V-FITC/PI Apoptosis Detection Kit

Preparation and Storage

Store at 4°C for up to one year. Annexin V-FITC and PI Staining Solution should be stored in dark.
Cautions:
1. For maximal assay performance, this kit should be used within 3-6 months. Avoid freeze / thaw cycles.
2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.
4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Related Products

Annexin V-FITC Reagent (MBS2557006)
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Annexin V-PE Reagent (MBS2557008)
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Annexin V-EGFP Reagent (MBS2557010)
Annexin V Binding Buffer(10 ×) (MBS2557011)
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Annexin V-AF647 / 7-AAD Apoptosis Detection Kit (MBS2557017)
Annexin V-PE / 7-AAD Apoptosis Detection Kit (MBS2557018)
Annexin V-APC / PI Apoptosis Detection Kit (MBS2557019)
Annexin V-APC / 7-AAD Apoptosis Detection Kit (MBS2557020)
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Instructions

The Annexin V Binding Buffer (10 ×)[Cat: MBS2557011 ] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.
For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.

Staining Procedure

1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures and centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells, resuspend gently and count the cells.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!
2. Split the cell suspension into tubes, 1-5 × 10^5 cells for each. Centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 uL of 1 × Annexin V Binding Buffer to resuspend the cells.
3. Add 5 ul of Annexin V-FITC and 5 ul of Propidium Iodide (PI) Staining Solution to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.

Product Categories/Family for Annexin V kit

Apoptosis Detection Kit

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for Annexin V kit

Apoptosis

Other Notes

Small volumes of Annexin V kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Annexin V purchase

MBS2557014 is a kit targeting Annexin V. Also known as Annexin V-FITC/PI Apoptosis Detection Kit.

Additional Details

NCBI/Uniprot data below describe general gene information for Annexin V. It may not necessarily be applicable to this product.

Protein Family

Annexin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.