Leishmania IgG (IgG) ELISA Kit for Human

Background: Leishmaniasis is an infectious disease, caused by protozoic parasites of the genus Leishmania. The disease occurs predominantly in tropical and subtropical climate zones. Primarily affected are farm and domestic animals, however the disease can also be transmitted to humans. Leishmaniasis is distributed worldwide with high incidences in Eastern Africa, Southern America and Asia. In Europe, the Mediterranean area is particularly affected. Annually, some 1.5 million cases of cutaneous and another 0.5 million cases of visceral leishmaniases are reported. Around 12 million people are infected with Leishmania worldwide. Dogs and rodents serve as the main reservoirs for Leishmania, but also cats, horses, sheep and cattle may be afflicted. Sandflies (phlebotominae) or other moth flies (psychodidae) transmit the parasites to humans. The incubation period is very variable, ranging from a few weeks up to several years. ELISA test systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.

Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.

Intended Uses: The Leishmania IgG ELISA test is a qualitative immunoassay for the demonstration of IgG antibodies in serum or plasma directed against Leishmania. The assay is recommended for the sensitive detection of such antibodies in various kinds of samples.

Small volumes of IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495326 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leishmania IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgG. The ELISA analytical biochemical technique of the MBS495326 kit is based on IgG antibody-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).