Podocalyxin (PCX) ELISA Kit for Human

Name: Human PCX ELISA Kit; Podocalyxin; MyBioSource
Brand: MyBioSource
SKU: MBS2023254
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2023254

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin (PCX). Standards or samples are then added to the appropriate microtiter plate wells… More details

$405 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Podocalyxin (PCX), ELISA Kit

Full Product Name

Human Podocalyxin (PCX) ELISA Kit

Product Gene Name

PCX elisa kit

Product Synonym Names

Gp200; PCLP; PC; PODXL; Podocalyxin Like Protein 1; GCTM-2 antigen

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Urine, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.054ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Podocalyxin (PCX).
No significant cross-reactivity or interference between Podocalyxin (PCX) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

55,386 Da

OMIM

602632

3D Structure

ModBase 3D Structure for O00592

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Podocalyxin (PCX) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Podocalyxin (PCX) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for PCX elisa kit

Tumor immunity; Hematology; Kidney biomarker

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PCX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCX purchase

MBS2023254 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podocalyxin (PCX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCX. The ELISA analytical biochemical technique of the MBS2023254 kit is based on PCX antibody-PCX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PCX. It may not necessarily be applicable to this product.

NCBI GI #

66277202

NCBI GeneID

5420

NCBI Accession #

NP_001018121.1

UniProt Primary Accession #

O00592

UniProt Secondary Accession #

Q52LZ7; Q53ER6; A6NHX8

UniProt Related Accession #

O00592

GenBank Nucleotide #

NM_001018111.2

NCBI Official Full Name

podocalyxin isoform 1

NCBI Official Synonym Full Names

podocalyxin-like

NCBI Official Symbol

PODXL

NCBI Official Synonym Symbols

PC; PCLP; Gp200; PCLP-1

UniProt Protein Name

Podocalyxin

UniProt Synonym Protein Names

GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1

Protein Family

Protein pecanex

UniProt Gene Name

PODXL

UniProt Synonym Gene Names

PCLP; PCLP1; PC; PCLP-1

UniProt Entry Name

PODXL_HUMAN

NCBI Protein Information

podocalyxin

NCBI Summary for PCX

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

UniProt Comments for PCX

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: ruffle; extracellular space; microvillus membrane; integral to plasma membrane; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane; filopodium; lipid raft

Molecular Function: protein binding

Biological Process: cell migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; regulation of microvillus biogenesis; positive regulation of cell migration

Research Articles on PCX

1. These findings show that overexpression of PODXL enhanced invadopodia formation and tumor metastasis by inducing Rac1/Cdc42/cortactin signaling network.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PCX elisa kit

Reference Product	PubMed Publications
CD34 elisa kit	>12 publications with PCX and CD34

Diseases associated with PCX elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>10 publications with PCX and Kidney Diseases
Liver Neoplasms Antibodies	>5 publications with PCX and Liver Neoplasms
Liver Diseases Antibodies	>4 publications with PCX and Liver Diseases
Breast Neoplasms Antibodies	>2 publications with PCX and Breast Neoplasms
Cardiovascular Diseases Antibodies	>2 publications with PCX and Cardiovascular Diseases
Brain Diseases Antibodies	>2 publications with PCX and Brain Diseases
Disease Models, Animal Antibodies	>2 publications with PCX and Disease Models, Animal
Ovarian Neoplasms Antibodies	>2 publications with PCX and Ovarian Neoplasms
Heart Diseases Antibodies	>2 publications with PCX and Heart Diseases
Proteinuria Antibodies	>2 publications with PCX and Proteinuria

Organs/Tissues associated with PCX elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>20 publications with PCX and Kidney
Vascular Antibodies	>14 publications with PCX and Vascular
Blood Antibodies	>14 publications with PCX and Blood
Bone Antibodies	>11 publications with PCX and Bone
Embryonic Tissue Antibodies	>9 publications with PCX and Embryonic Tissue
Lung Antibodies	>5 publications with PCX and Lung
Liver Antibodies	>5 publications with PCX and Liver
Heart Antibodies	>5 publications with PCX and Heart
Muscle Antibodies	>4 publications with PCX and Muscle
Brain Antibodies	>4 publications with PCX and Brain