Brdu Kit

Background: A non-isotopic immunohistochemical staining procedure for the localization and quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. BrdU Immunohistochemistry Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and visual staining (dark brown nuclei) of these cells which is achieved using a Prediluted Biotinylated Sheep anti-BrdU Detector Antibody followed by Streptavidin-HRP Conjugate and DAB (diaminobenzidine) substrate.

Intended Uses: The BrdU Immunohistochemistry Kit is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

Small volumes of BrdU kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS574011 is a kit targeting BrdU. Also known as BrdU Immunohistochemistry Kit.