Interleukin 21 (IL21) Active Protein

Name: IL21 Active Protein; Interleukin 21; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650660
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650660

Interleukin-21 (IL-21), expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines, which includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15. Its private receptor, IL-21R, is expressed on T, B, and NK cells.… More details

$480 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 21 (IL21), Active Protein

Full Product Name

Interleukin 21, Recombinant, Human (IL-21)

Product Gene Name

IL21 active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Gly-Gl n-Asp-Arg.

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Purity/Purification

Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver stained gel. Endotoxin: 0.1ng/ug (IEU/ug).

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O, 0.1% HSA or BSA,,aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

15.46kD

Chromosome Location

Chromosome: 4; NC_000004.11 (123533783..123542211, complement). Location: 4q26-q27

OMIM

605384

3D Structure

ModBase 3D Structure for Q9HBE4

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

The ED50=1-10ng/ml as determined by the dose-dependent proliferation of human peripheral blood mononuclear cells is < 5ng/ml.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.54 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-21 as a Reference Standard.

Product Categories/Family for IL21 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL21 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL21 purchase

MBS650660 is an active protein targeting IL21. Also known as Interleukin 21, Recombinant, Human (IL-21). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL21. It may not necessarily be applicable to this product.

NCBI GI #

11093536

NCBI GeneID

59067

UniProt Primary Accession #

Q9HBE4

UniProt Secondary Accession #

A5J0L4

UniProt Related Accession #

Q9HBE4

NCBI Official Full Name

interleukin 21

NCBI Official Synonym Full Names

interleukin 21

NCBI Official Symbol

IL21

NCBI Official Synonym Symbols

Za11; IL-21

UniProt Protein Name

Interleukin-21

UniProt Synonym Protein Names

Za11

Protein Family

Interleukin

UniProt Gene Name

IL21

UniProt Entry Name

IL21_HUMAN

NCBI Protein Information

interleukin-21; OTTHUMP00000164088; interleukin-21 isoform

NCBI Summary for IL21

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for IL21

Function: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells

By similarity. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Ref.1 Ref.6

Subcellular location: Secreted Ref.1.

Tissue specificity: Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. Ref.1

Sequence similarities: Belongs to the IL-15/IL-21 family.

Sequence caution: The sequence AAG29348.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66258.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66259.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66260.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66261.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66262.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH69124.1 differs from that shown. Reason: Erroneous initiation. The sequence AAU88182.1 differs from that shown. Reason: Erroneous initiation. The sequence ABG36529.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on IL21

1. These results indicate that IL-21 regulates B cell development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL21 active protein

Reference Product	PubMed Publications
IL21R active protein	>228 publications with IL21 and IL21R

Pathways associated with IL21 active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with IL21 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>106 publications with IL21 and Neoplasms
Inflammation Antibodies	>86 publications with IL21 and Inflammation
Lupus Erythematosus, Systemic Antibodies	>34 publications with IL21 and Lupus Erythematosus, Systemic
Lymphoma Antibodies	>31 publications with IL21 and Lymphoma
Celiac Disease Antibodies	>21 publications with IL21 and Celiac Disease
Leukopenia Antibodies	>6 publications with IL21 and Leukopenia
Thyroid Diseases Antibodies	>6 publications with IL21 and Thyroid Diseases
Mammary Neoplasms, Experimental Antibodies	>4 publications with IL21 and Mammary Neoplasms, Experimental
Thrombocytopenia Antibodies	>3 publications with IL21 and Thrombocytopenia
Dermatitis, Atopic Antibodies	>3 publications with IL21 and Dermatitis, Atopic