GFP Vector – GFP Lentivirus Control (GFP Lentivirus)

• Provided as 200 uL of lentiviral supernatant at >1 x 10^6 TU/mL

Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes and macrophages, cell types that previous retrovirus vectors could not be used. Lentiviral vectors have also proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. Recently, the lentivirus system is widely used to integrate siRNA efficiently in a wide variety of cell lines and primary cells both in vitro and in vivo.

The provided GFP lentivirus control is a VSVG-pseudotyped pantropic virus capable of infecting both dividing and non-dividing cells. The virus contains GFP and blasticidin resistant genes (Figure 1), it provides a useful control for transduction. This control virus can also be used to generate GFP stable cell lines, and stable clones can be selected by green fluorescence sorting.

1. Naldini, L., U. Blomer, P. Gallay, D. Ory, R. Mulligan, F. H. Gage, I. M. Verma, and D. Trono (1996) Science 272, 263-267.
2. Verma, I. M., and N. Somia (1997) Nature 389, 239-242
3. Kahl C. A., Marsh J., Fyffe J., Sanders D. A., and K. Cornetta (2004) J Virol. 78:1421-30.
4. White S. M., Renda M., Nam N. Y., Klimatcheva E., Zhu Y., Fisk J., Halterman M., Rimel B. J., Federoff H., Pandya S., Rosenblatt J. D., and V. Planelles (1999) J Virol. 73:2832-40.
5. Kafri T., van Praag H., Ouyang L., Gage F. H., and I. M. Verma (1999) J Virol. 73:576-84.

Small volumes of GFP Lentivirus Control vector vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168880 is a vector targeting GFP Lentivirus Control.