T4 DNA Ligase Enzyme

Name: Enzyme; T4 DNA Ligase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS9141679
Price: 195 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9141679

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and… More details

$195 / 16K Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

T4 DNA Ligase, Enzyme

Full Product Name

T4 DNA Ligase

Host

E Coli

Form/Format

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 at 25 degree C

Concentration

400,000 U/ml (lot specific)

Preparation and Storage

Store at -20 degree C.

Source Note

An E Coli strain that carries the T4 DNA ligase gene.

Unit Definition

One unit is defined as the amount of enzyme required to give 50% ligase of HindIII fragments of lambda DNA (5' DNA termini concentration of 0.12 muM, 300- mug/ml) in a total reaction volume of 20 mul in 30 minutes at 16 degree C in 1X T4 DNA Ligase Reaction Buffer.

Reaction Conditions

1X T4 DNA Ligase Reaction Buffer.

Reaction Buffer or Mix

1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl,10 mM MgCl2,10 mM DTT,1 mM ATP,pH7.5 at 25 degree C

Heat Inactivation

65 degree C for 10 min.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Applications Tested/Suitable for T4 DNA Ligase enzyme

Cloning Ligation

Other Notes

Small volumes of T4 DNA Ligase enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for T4 DNA Ligase purchase

MBS9141679 is an enzyme targeting T4 DNA Ligase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for T4 DNA Ligase. It may not necessarily be applicable to this product.

Protein Family

DNA ligase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.