Melatonin ELISA Kits

Melatonin (N-acetyl-5-methoxytrypamine) is an important hormone involved in the regulation of sleep: wake cycles. After entering circulation melatonin acts as endocrine factor and a chemical messenger of light and darkness (circadian and circannual pacemaker). Melatonin is synthesized by pinealocytes in the pineal gland and in retinal photoreceptors. Although analytical methods of melatonin determination such as gas chromatography–mass spectrometry (GC–MS) and high performance liquid chromatography (HPLC) are available they limittions such as high cost of apparatus, complex sample preparation, large sample volume, and are not suitable for measuring large numbers of samples.

Addressing these issues and in contrast enzyme immunoassay (ELISA) methods have been developed to quantify melatonin enabling endocrinologists and researchers to process many biological samples in 4 easy steps. 1) sample/conjugate incubation, 2) plate washing, 3) color reaction and 4) reading of results. Melatonin ELISA Kits sourced by MyBioSource is a competitive for of ELISA and it involves the simultaneous addition of competing antibodies. The melatonin antibody is coated on 96-well plates as the solid phase, and the enzyme-labeled melatonin is mixed with sample melatonin to compete for the antibody. The ratio of sample melatonin versus enzyme-labeled melatonin determines the intensity of the color reaction. This assay can directly measure melatonin in the cell culture medium. Melatonin ELISA kits sourced by MyBioSource can be applied in multiple labs rapidly with low cost compared with other methods.