Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MAdCAM-1) ELISA Kit for Monkey

Intended Uses: This M6PR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse M6PR. This ELISA kit for research use only!

Principle of the Assay: M6PR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-M6PR antibody and an M6PR-HRP conjugate. The assay sample and buffer are incubated together with M6PR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the M6PR concentration since M6PR from samples and M6PR-HRP conjugate compete for the anti-M6PR antibody binding site. Since the number of sites is limited, as more sites are occupied by M6PR from the sample, fewer sites are left to bind M6PR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The M6PR concentration in each sample is interpolated from this standard curve.

Small volumes of MAdCAM-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS735007 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAdCAM-1. The ELISA analytical biochemical technique of the MBS735007 kit is based on MAdCAM-1 antibody-MAdCAM-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAdCAM-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAdCAM-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).