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CTLA4 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751897

Rabbit IgG Monoclonal antibody for P16410 detection. Tested positive for WB IF/IF in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CTLA4 (CD152), Monoclonal Antibody
Full Product Name
Anti-CTLA4 (CD152) Monoclonal Antibody
Product Gene Name
anti-CTLA4 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
6,560 Da
3D Structure
ModBase 3D Structure for P16410
Immunogen
A synthesized peptide derived from human CTLA4 (CD152) Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CTLA4 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CTLA4 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-CTLA4 antibody

Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (M00020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTLA4.
Rabbit Anti-CTLA4 Monoclonal Antibody – Cat# MBS1751897

Other Notes

Small volumes of anti-CTLA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CTLA4 purchase

MBS1751897 is an antibody targeting CTLA4. Also known as Anti-CTLA4 (CD152) Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CTLA4. It may not necessarily be applicable to this product.

NCBI GI #
83700231
NCBI GeneID
1493
NCBI Accession #
NP_001032720.1
UniProt Primary Accession #
P16410
UniProt Secondary Accession #
O95653; Q0PP65; Q52MC1; Q53TD5; Q5S005; Q8WXJ1; Q96P43; Q9UKN9; A0N1S0; E9PDH0
UniProt Related Accession #
P16410
GenBank Nucleotide #
NM_001037631.3
NCBI Official Full Name
cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM
NCBI Official Synonym Full Names
cytotoxic T-lymphocyte associated protein 4
NCBI Official Symbol
CTLA4
NCBI Official Synonym Symbols
CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
UniProt Protein Name
Cytotoxic T-lymphocyte protein 4
UniProt Synonym Protein Names
Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD_antigen: CD152
Protein Family
Cytotoxic T-lymphocyte protein
UniProt Gene Name
CTLA4
UniProt Synonym Gene Names
CD152; CTLA-4
NCBI Protein Information
cytotoxic T-lymphocyte protein 4

NCBI Summary for CTLA4

This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for CTLA4

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Research Articles on CTLA4

1. Paget disease is characterized by an intense lymphocytic response, devoid of the immune-suppressive impact of the PD-L1 pathway, but with occasional CTLA-4 expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CTLA4 antibody

 Reference Product  PubMed Publications
 CD80 antibody  >976 publications with CTLA4 and CD80
 CD86 antibody  >620 publications with CTLA4 and CD86
 FOXP3 antibody  >535 publications with CTLA4 and FOXP3
 LCK antibody  >18 publications with CTLA4 and LCK
 PTPN11 antibody  >13 publications with CTLA4 and PTPN11
 CD276 antibody  >9 publications with CTLA4 and CD276
 FYN antibody  >7 publications with CTLA4 and FYN
 LYN antibody  >4 publications with CTLA4 and LYN

Pathways associated with anti-CTLA4 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-CTLA4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >805 publications with CTLA4 and Neoplasms
 Melanoma Antibodies  >396 publications with CTLA4 and Melanoma
 Necrosis Antibodies  >339 publications with CTLA4 and Necrosis
 Inflammation Antibodies  >329 publications with CTLA4 and Inflammation
 Arthritis, Rheumatoid Antibodies  >206 publications with CTLA4 and Arthritis, Rheumatoid
 Graves Disease Antibodies  >167 publications with CTLA4 and Graves Disease
 Carcinoma Antibodies  >148 publications with CTLA4 and Carcinoma
 Neoplasms, Experimental Antibodies  >132 publications with CTLA4 and Neoplasms, Experimental
 Liver Diseases Antibodies  >131 publications with CTLA4 and Liver Diseases
 Lymphoma Antibodies  >78 publications with CTLA4 and Lymphoma

Organs/Tissues associated with anti-CTLA4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2910 publications with CTLA4 and Blood
 Lymph Node Antibodies  >227 publications with CTLA4 and Lymph Node
 Kidney Antibodies  >181 publications with CTLA4 and Kidney
 Lung Antibodies  >168 publications with CTLA4 and Lung
 Prostate Antibodies  >68 publications with CTLA4 and Prostate
 Mammary Gland Antibodies  >1 publications with CTLA4 and Mammary Gland
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