PCR Kits - Ready-to-Use Polymerase Chain Reaction Reagents

MyBioSource provides a comprehensive range of ready-to-use PCR kits for researchers worldwide. Our selection includes high-quality PCR reagents and complete PCR reagent kits designed for a variety of applications, from pathogen detection to genetic analysis. Whether you need conventional kits or advanced real-time solutions, you'll find reliable PCR products to accelerate your research.

What is a Polymerase Chain Reaction Kit?

The powerful polymerase chain reaction (PCR) molecular biology technique enables scientists to hone in on a segment of DNA and copy it thousands, millions, or even billions of times over. This can be accomplished relatively easily and inexpensively. It is incredible that a single copy or just a few copies of a DNA molecular sequence can be amplified into thousands or more copies of the same DNA molecule.

Since it was first developed in 1983 by Kary Mullis, who won the Nobel Prize in Chemistry in 1993 for his invention, PCR has become an invaluable molecular biology technique. Today, polymerase chain reaction kits are used worldwide for a variety of applications, including the identification of infectious agents, disease diagnosis, and cloning DNA fragments.

In conventional PCR, the amount of accumulated PCR product is measured at the end of the reaction. While this approach doesn't allow for determining the starting concentration of the target DNA in real- time, the amplified PCR product can be run on an agarose gel and compared to standards to obtain a semi-quantitative result. Conventional PCR is widely used for cloning, sequencing, and genotyping.

Real-Time qPCR and RT-PCR Kits

In contrast to traditional methods, real-time PCR, also known as quantitative polymerase chain reaction (qPCR), measures the amplification of the target DNA molecule as the reaction is occurring. Data is collected during the exponential phase when PCR products are doubling at every cycle. During this phase, the quantity of the PCR product is directly proportional to the amount of starting template DNA, making qPCR one of the most sensitive genomic analysis techniques available.

Another variation is the reverse transcription polymerase chain reaction (RT-PCR), which is used to detect gene expression by generating complementary DNA (cDNA) from an RNA template. The cDNA is then amplified using the PCR reagents in the kit. When combined with qPCR, this technique is known as qRT- PCR.

Optimized PCR Reagents for Reliable Results

As a leading provider of PCR solutions, MyBioSource ensures our ready-to-use PCR reagent kits are popular among scientists because the design, including primer design, positive controls, and other kit components, has already been optimized for detection and performance.

The methodology is detailed in each kit manual. For many of our qPCR kits, detection is based on the fluorogenic 5' nuclease assay. In this process, a thermostable DNA polymerase cleaves a primer-probe to separate a reporter dye from a quencher dye. This generates a fluorescent signal that is monitored in real- time and is directly proportional to the amount of PCR product being generated.

Applications and System Compatibility

Our PCR kits are designed for a wide range of critical applications, including:

• Infectious diseases
• Animal pathogens
• Tumor detection
• Genetic diseases
• Monitoring pathogens associated with organ transplantation

Many of our PCR kits are CE marked and compliant with ISO13485 standards. They are compatible with most brands of real-time PCR systems, including those from Roche, Bio-Rad, and Applied Biosystems.

Frequently Asked Questions about PCR Kits

What is included in a typical PCR kit?

Most PCR kits are sold as complete PCR reagent kits. They typically include essential PCR reagents such as a DNA polymerase, dNTPs, reaction buffers, and primers specific to the target sequence. Our ready-to- use kits are fully optimized to ensure reliable amplification and consistent generation of PCR products.

What are the main uses for polymerase chain reaction kits?

Polymerase chain reaction kits are fundamental tools in molecular biology used for a wide range of applications. These include genotyping, DNA sequencing, disease diagnosis, pathogen detection, and gene expression analysis. They are essential for any research that requires amplifying specific DNA or RNA sequences.

How are PCR products detected?

PCR products (the amplified DNA segments) can be detected in several ways. In conventional PCR, they are often visualized as bands on an agarose gel. In real-time PCR (qPCR), fluorescence-based PCR reagents are used to monitor the amplification in real-time, allowing for quantification of the PCR products as they are created.

Are your PCR reagent kits compatible with different machine brands?

Yes. Our PCR reagent kits are designed for broad compatibility with the most common real-time PCR systems used in laboratories today, including systems from major brands like Roche, Bio-Rad, and Applied Biosystems. Please check the individual product manual for any specific system requirements.