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Discs (DLG5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2033859
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MBS2033859 is an antibody targeting DLG5. Also known as Polyclonal Antibody to Discs, Large Homolog 5 (DLG5). Reactive in Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Discs (DLG5), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Discs, Large Homolog 5 (DLG5)
Product Gene Name
anti-DLG5 antibody
Product Synonym Names
LP-DLG; P-DLG5; PDLG; Discs large protein P-dlg; Placenta and prostate DLG
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against DLG5. It has been selected for its ability to recognize DLG5 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: DLG5 (MBS2033859)
Immunogen: DLG5 (MBS2034310)
Matching Pairs
Unconjugated Antibody: DLG5 (MBS2033859)
APC-CY7 Conjugated Antibody: DLG5 (MBS2069761)
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Molecular Weight
214,386 Da
3D Structure
ModBase 3D Structure for E9Q9R9
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
DLG5 (Asp1724~Ser1917)
Conjugate
No Conjugate
Cross Reactivity
Human, Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant DLG5 (Asp1724~Ser1917) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069763

Product Categories/Family for anti-DLG5 antibody

Knock-Out Validation Antibody
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-DLG5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-DLG5 antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-DLG5 antibody

Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-DLG5 Polyclonal Antibody – Cat# MBS2033859

Western Blot (WB) of anti-DLG5 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-DLG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2033859

Immunohistochemistry (IHC) of anti-DLG5 antibody

DAB staining on fromalin fixed paraffin- embedded brain tissue)
Rabbit Anti-DLG5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2033859

Knockout Validation of anti-DLG5 antibody

Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
Rabbit Anti-DLG5 Polyclonal Antibody, Knockout Validation – Cat# MBS2033859

Other Notes

Small volumes of anti-DLG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DLG5. It may not necessarily be applicable to this product.

NCBI GI #
254588083
NCBI GeneID
71228
NCBI Accession #
NP_001156985.1
UniProt Primary Accession #
E9Q9R9
UniProt Secondary Accession #
Q3UGX5
UniProt Related Accession #
E9Q9R9
GenBank Nucleotide #
NM_001163513.1
NCBI Official Full Name
disks large homolog 5 isoform 1
NCBI Official Synonym Full Names
discs large MAGUK scaffold protein 5
NCBI Official Symbol
Dlg5
NCBI Official Synonym Symbols
T25557; mKIAA0583; 4933429D20Rik
UniProt Protein Name
Disks large homolog 5
Protein Family
Disks large
UniProt Gene Name
Dlg5
NCBI Protein Information
disks large homolog 5

UniProt Comments for DLG5

DLG5: May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton. Belongs to the MAGUK family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14|14 A3

Cellular Component: cell junction; cell projection; cell-cell adherens junction; cytoplasm; cytoskeleton; membrane; plasma membrane; postsynaptic density; postsynaptic membrane; synapse

Molecular Function: beta-catenin binding; cytoskeletal protein binding; protein binding

Biological Process: apical protein localization; epithelial to mesenchymal transition; establishment or maintenance of epithelial cell apical/basal polarity; maintenance of cell polarity; midbrain development; negative regulation of cell migration; negative regulation of T cell proliferation; polarized epithelial cell differentiation; positive regulation of smoothened signaling pathway; positive regulation of synaptogenesis; protein complex assembly; regulation of apoptosis; zonula adherens assembly
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Research Articles on DLG5

1. Dlg5 is required for Hh-induced enrichment of Kif7 and Gli2 at the tip of the cilium but is dispensable for Gpr161 exit from the cilium and the consequent suppression of Gli3 processing into its repressor form

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-DLG5 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with DLG5 and Inflammation
 Necrosis Antibodies  >6 publications with DLG5 and Necrosis
 Adenocarcinoma Antibodies  >3 publications with DLG5 and Adenocarcinoma
 Lung Neoplasms Antibodies  >3 publications with DLG5 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with DLG5 and Cell Transformation, Neoplastic
 Lung Diseases Antibodies  >2 publications with DLG5 and Lung Diseases
 Abnormalities, Multiple Antibodies  >2 publications with DLG5 and Abnormalities, Multiple
 Neoplasm Metastasis Antibodies  >2 publications with DLG5 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >1 publications with DLG5 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with DLG5 and Cardiovascular Diseases

Organs/Tissues associated with anti-DLG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with DLG5 and Brain
 Muscle Antibodies  >6 publications with DLG5 and Muscle
 Lung Antibodies  >5 publications with DLG5 and Lung
 Intestine Antibodies  >5 publications with DLG5 and Intestine
 Kidney Antibodies  >3 publications with DLG5 and Kidney
 Skin Antibodies  >2 publications with DLG5 and Skin
 Bone Antibodies  >1 publications with DLG5 and Bone
 Ovary Antibodies  >1 publications with DLG5 and Ovary
 Pancreas Antibodies  >1 publications with DLG5 and Pancreas
 Embryonic Tissue Antibodies  >1 publications with DLG5 and Embryonic Tissue
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