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Fibrillarin (FBL) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2034010
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MBS2034010 is an antibody targeting FBL. Also known as Polyclonal Antibody to Fibrillarin (FBL). Reactive in Human, Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibrillarin (FBL), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Fibrillarin (FBL)
Product Gene Name
anti-FBL antibody
Product Synonym Names
FIB; FLRN; RNU3IP1; rRNA 2'-O-Methyltransferase Fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against FBL. It has been selected for its ability to recognize FBL in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: FBL (MBS2034010)
Immunogen: FBL (MBS2034141)
Matching Pairs
Unconjugated Antibody: FBL (MBS2034010)
APC-CY7 Conjugated Antibody: FBL (MBS2066109)
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Molecular Weight
34,222 Da
3D Structure
ModBase 3D Structure for P22509
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Fragment
FBL (Gly85~Cys274)
Conjugate
No Conjugate
Cross Reactivity
Human, Rat
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant FBL (Gly85~Cys274) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066111

Product Categories/Family for anti-FBL antibody

Knock-Out Validation Antibody
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-FBL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-FBL antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-FBL antibody

Western Blot: Sample: Human A375 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat FBL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-FBL Polyclonal Antibody – Cat# MBS2034010

Western Blot (WB) of anti-FBL antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-FBL Polyclonal Antibody, Western Blot (WB) – Cat# MBS2034010

Immunohistochemistry (IHC) of anti-FBL antibody

DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
Rabbit Anti-FBL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2034010

Knockout Validation of anti-FBL antibody

Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: FBL knockout K562 cell lysate; Predicted MW: 34kd Observed MW: 34kd Primary Ab: 3ug/ml Rabbit Anti-Rat FBL Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
Rabbit Anti-FBL Polyclonal Antibody, Knockout Validation – Cat# MBS2034010

Other Notes

Small volumes of anti-FBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FBL. It may not necessarily be applicable to this product.

NCBI GI #
71043704
NCBI GeneID
292747
NCBI Accession #
NP_001020814.1
UniProt Primary Accession #
P22509
UniProt Secondary Accession #
Q4KLH8
UniProt Related Accession #
P22509
GenBank Nucleotide #
NM_001025643.1
NCBI Official Full Name
rRNA 2'-O-methyltransferase fibrillarin
NCBI Official Synonym Full Names
fibrillarin
NCBI Official Symbol
Fbl
UniProt Protein Name
rRNA 2'-O-methyltransferase fibrillarin
UniProt Synonym Protein Names
Histone-glutamine methyltransferase; Nucleolar protein 1
Protein Family
rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name
Fbl
NCBI Protein Information
rRNA 2'-O-methyltransferase fibrillarin

UniProt Comments for FBL

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: EC 2.1.1.-; Methyltransferase; Nucleolus; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: Cajal body; chromosome; dense fibrillar component; fibrillar center; granular component; membrane; nucleolus; nucleoplasm; nucleus; small-subunit processome

Molecular Function: ATPase binding; protein binding; RNA binding; rRNA methyltransferase activity

Biological Process: box C/D snoRNA 3'-end processing; osteoblast differentiation; rRNA methylation; snoRNA localization; snoRNA metabolic process; tRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FBL antibody

 Reference Product  PubMed Publications
 SNU13 antibody  >3 publications with FBL and SNU13
 NOP56 antibody  >3 publications with FBL and NOP56

Pathways associated with anti-FBL antibody

 Products by Pathway  Pathway Diagram
 Ribosome Biogenesis In Eukaryotes Pathway antibodies  Ribosome Biogenesis In Eukaryotes Pathway Diagram
 Ribosome Biogenesis In Eukaryotes Pathway antibodies  Ribosome Biogenesis In Eukaryotes Pathway Diagram
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