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DIO3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9205926

DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites,… More details

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Product Overview

Online Datasheet in PDF
Product Name
DIO3, Polyclonal Antibody
Full Product Name
DIO3 Antibody (C-term)
Product Gene Name
anti-DIO3 antibody
Product Synonym Names
Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase; DIO3; ITDI3; TXDI3
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.5 (lot specific)
Specificity
This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
DIO3 peptide (MBS9228259) is used for blocking the activity of DIO3 antibody (MBS9205926)
Molecular Weight
33947
Immunogen Sequence Positions
250-278
OMIM
601038
3D Structure
ModBase 3D Structure for P55073
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN

Product Categories/Family for anti-DIO3 antibody

Cancer; Metabolism; Neuroscience; Signal transduction
Applications Tested/Suitable for anti-DIO3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-DIO3 antibody
WB~~1:1000

Western Blot (WB) of anti-DIO3 antibody

Western blot analysis of DIO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.
Rabbit Anti-DIO3 Polyclonal Antibody – Cat# MBS9205926

Immunohistochemistry (IHC) of anti-DIO3 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-DIO3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9205926

Product References and Citations for anti-DIO3 antibody

Bessho, K., et al. Eur. J. Pediatr. 169(2):215-221(2010)
Wallace, C., et al. Nat. Genet. 42(1):68-71(2010)
Aerts, G., et al. Endocrinology 150(11):5171-5180(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 94(5):1623-1629(2009)
Panicker, V., et al. J. Clin. Endocrinol. Metab. 93(8):3075-3081(2008)

Other Notes

Small volumes of anti-DIO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DIO3 purchase

MBS9205926 is an antibody targeting DIO3. Also known as DIO3 Antibody (C-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DIO3. It may not necessarily be applicable to this product.

NCBI GI #
255958313
NCBI GeneID
1735
NCBI Accession #
NP_001353.4
UniProt Primary Accession #
P55073
UniProt Secondary Accession #
Q8WVN5; G3XAM0
UniProt Related Accession #
P55073
GenBank Nucleotide #
NM_001362.3
NCBI Official Full Name
type III iodothyronine deiodinase
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type III
NCBI Official Symbol
DIO3
NCBI Official Synonym Symbols
D3; 5DIII; TXDI3; DIOIII
UniProt Protein Name
Type III iodothyronine deiodinase
UniProt Synonym Protein Names
5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase
Protein Family
Thyroxine 5-deiodinase
UniProt Gene Name
DIO3
UniProt Synonym Gene Names
ITDI3; TXDI3
UniProt Entry Name
IOD3_HUMAN
NCBI Protein Information
type III iodothyronine deiodinase

NCBI Summary for DIO3

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
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UniProt Comments for DIO3

DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: EC 1.97.1.11; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: integral to membrane; plasma membrane; endosome membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: thyroid hormone catabolic process; thyroid hormone generation; positive regulation of multicellular organism growth; hormone biosynthetic process
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Research Articles on DIO3

1. the microRNA cluster at the Dlk1-Dio3 locus is upregulated in lung adenocarcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DIO3 antibody

 Reference Product  PubMed Publications
 DLK1 antibody  >105 publications with DIO3 and DLK1
 RTL1 antibody  >15 publications with DIO3 and RTL1
 TRH antibody  >3 publications with DIO3 and TRH
 SLC16A2 antibody  >1 publications with DIO3 and SLC16A2

Pathways associated with anti-DIO3 antibody

 Products by Pathway  Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram

Diseases associated with anti-DIO3 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >8 publications with DIO3 and Liver Diseases
 Liver Neoplasms Antibodies  >6 publications with DIO3 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with DIO3 and Carcinoma, Hepatocellular
 Heart Diseases Antibodies  >5 publications with DIO3 and Heart Diseases
 Hypertrophy Antibodies  >5 publications with DIO3 and Hypertrophy
 Prenatal Exposure Delayed Effects Antibodies  >4 publications with DIO3 and Prenatal Exposure Delayed Effects
 Prenatal Injuries Antibodies  >4 publications with DIO3 and Prenatal Injuries
 Fetal Growth Retardation Antibodies  >3 publications with DIO3 and Fetal Growth Retardation
 Disease Models, Animal Antibodies  >3 publications with DIO3 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >3 publications with DIO3 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-DIO3 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >31 publications with DIO3 and Liver
 Placenta Antibodies  >14 publications with DIO3 and Placenta
 Testis Antibodies  >9 publications with DIO3 and Testis
 Adipose Tissue Antibodies  >7 publications with DIO3 and Adipose Tissue
 Uterus Antibodies  >7 publications with DIO3 and Uterus
 Stomach Antibodies  >3 publications with DIO3 and Stomach
 Intestine Antibodies  >1 publications with DIO3 and Intestine
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