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ALPK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604868

MBS9604868 is an antibody targeting ALPK1. Also known as ALPK1 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALPK1, Polyclonal Antibody
Full Product Name
ALPK1 Antibody
Product Gene Name
anti-ALPK1 antibody
Product Synonym Names
8430410J10Rik; Alpha protein kinase 1; Alpha-protein kinase 1; ALPK1; ALPK1_HUMAN; Chromosome 4 kinase; LAK; Lymphocyte alpha kinase; Lymphocyte alpha protein kinase; Lymphocyte alpha-protein kinase; RGD1304613
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientrific).
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ALPK1 peptide (MBS9623585) is used for blocking the activity of ALPK1 antibody (MBS9604868)
Molecular Weight
139 KD
3D Structure
ModBase 3D Structure for Q96QP1
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ALPK1, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ALPK1 antibody
Western Blot (WB), Immunohisotchemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-ALPK1 antibody
WB 1:500-1:1000
IHC 1:50-1:200
IF/ICC 1:100-1:500

The optimal dilutions should be determined by the end user.

IMPORTANT:
For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-ALPK1 antibody

Western blot analysis of extracts from HepG2 cells using ALPK1 antibody.
Rabbit Anti-ALPK1 Polyclonal Antibody – Cat# MBS9604868

Immunohistochemistry (IHC) of anti-ALPK1 antibody

MBS9604868 at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
Rabbit Anti-ALPK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9604868

Immunohistochemistry (IHC) of anti-ALPK1 antibody

MBS9604868 at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.
Rabbit Anti-ALPK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9604868

Other Notes

Small volumes of anti-ALPK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPK1. It may not necessarily be applicable to this product.

NCBI GI #
156151443
NCBI GeneID
80216
NCBI Accession #
NP_001095876.1
UniProt Primary Accession #
Q96QP1
UniProt Secondary Accession #
Q68CI9; Q6P9F9; Q6ZNK4; Q9P201; B4E3G1; F5H138
UniProt Related Accession #
Q96QP1
GenBank Nucleotide #
NM_001102406.1
NCBI Official Full Name
alpha-protein kinase 1 isoform 1
NCBI Official Synonym Full Names
alpha kinase 1
NCBI Official Symbol
ALPK1
NCBI Official Synonym Symbols
LAK; 8430410J10Rik
UniProt Protein Name
Alpha-protein kinase 1
UniProt Synonym Protein Names
Chromosome 4 kinase1 PublicationManual assertion based on opinion iniRef.1"Alpha-kinases: a new class of protein kinases with a novel catalytic domain."Ryazanov A.G., Pavur K.S., Dorovkov M.V.Curr. Biol. 9:R43-R45(1999) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ASP-565; ARG-642; ILE-732 AND THR-861.
UniProt Gene Name
ALPK1
UniProt Synonym Gene Names
KIAA15271 PublicationManual assertion based on opinion iniRef.6
NCBI Protein Information
alpha-protein kinase 1

NCBI Summary for ALPK1

This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for ALPK1

Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed:28877472, PubMed:28222186, PubMed:30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed:30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering proinflammatory NF-kappa-B signaling (PubMed:30111836). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed:27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed:15883161).
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Research Articles on ALPK1

1. Combined exposure to the four high-risk genotypes of ALPK1 and the uric-acid-related loci of ABCG2, SLC2A9, and SLC22A12 was associated with an increased gout risk and a high PPV for gout.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ALPK1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with ALPK1 and Neoplasms

Organs/Tissues associated with anti-ALPK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with ALPK1 and Kidney
 Bone Antibodies  >1 publications with ALPK1 and Bone
 Prostate Antibodies  >1 publications with ALPK1 and Prostate
 Blood Antibodies  >1 publications with ALPK1 and Blood
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