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Leukemia Inhibitory Factor (LIF) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628697

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial… More details

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Product Overview

Online Datasheet in PDF
Product Name
Leukemia Inhibitory Factor (LIF), Polyclonal Antibody
Full Product Name
Leukemia Inhibitory Factor (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)
Product Gene Name
anti-LIF antibody
Product Synonym Names
Anti -Leukemia Inhibitory Factor (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human LIF.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,008 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (30636442..30642796, complement). Location: 22q12.2
OMIM
159540
3D Structure
ModBase 3D Structure for P15018
Immunogen
Synthetic peptide corresponding to a aa79-109 from the center region of human LIF, conjugated to KLH.

Product Categories/Family for anti-LIF antibody

Antibodies; Abs to Growth Factors; Cytokines
Applications Tested/Suitable for anti-LIF antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-LIF antibody
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50

Western Blot (WB) of anti-LIF antibody

Western blot analysis of LIF Antibody (Center) in CEM cell line lysates (35ug/lane). LIF (arrow) was detected using the purified Pab.
Rabbit Anti-LIF Polyclonal Antibody – Cat# MBS628697

Western Blot (WB) of anti-LIF antibody

Western blot analysis of LIF (arrow) using rabbit polyclonal LIF Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LIF gene.
Rabbit Anti-LIF Polyclonal Antibody, Western Blot (WB) – Cat# MBS628697

Immunohistochemistry (IHC) of anti-LIF antibody

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-LIF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628697

Flow Cytometry (FC/FACS) of anti-LIF antibody

Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-LIF Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS628697

Other Notes

Small volumes of anti-LIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIF purchase

MBS628697 is an antibody targeting LIF. Also known as Leukemia Inhibitory Factor (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIF. It may not necessarily be applicable to this product.

NCBI GI #
178415
NCBI GeneID
3976
UniProt Primary Accession #
P15018
UniProt Secondary Accession #
Q52LZ2; B2RCW7
UniProt Related Accession #
P15018
NCBI Official Full Name
leukemia inhibitory factor
NCBI Official Synonym Full Names
leukemia inhibitory factor (cholinergic differentiation factor)
NCBI Official Symbol
LIF
NCBI Official Synonym Symbols
CDF; DIA; HILDA
UniProt Protein Name
Leukemia inhibitory factor
UniProt Synonym Protein Names
Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor
Protein Family
Leukemia inhibitory factor
UniProt Gene Name
LIF
UniProt Synonym Gene Names
HILDA
UniProt Entry Name
LIF_HUMAN
NCBI Protein Information
leukemia inhibitory factor; MLPLI; D factor; OTTHUMP00000198698; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation stimulating factor; differentiation-stimulating factor; differentiation inhibitory activity

NCBI Summary for LIF

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq]
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UniProt Comments for LIF

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of hormone secretion; multicellular organismal development; leukemia inhibitory factor signaling pathway; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of meiosis; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; stem cell maintenance; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of peptidyl-tyrosine phosphorylation; neuron development; immune response; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; alveolus development; embryo implantation
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Research Articles on LIF

1. The epigenetic up-regulation of the LIF gene likely play an important role in the development of breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIF antibody

 Reference Product  PubMed Publications
 LIFR antibody  >429 publications with LIF and LIFR
 IL6ST antibody  >221 publications with LIF and IL6ST

Pathways associated with anti-LIF antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies  Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram

Diseases associated with anti-LIF antibody

 Disease Name  Pubmed Publications
 Leukemia Antibodies  >2765 publications with LIF and Leukemia
 Neoplasms Antibodies  >646 publications with LIF and Neoplasms
 Leukemia, Myeloid Antibodies  >214 publications with LIF and Leukemia, Myeloid
 Inflammation Antibodies  >213 publications with LIF and Inflammation
 Carcinoma Antibodies  >174 publications with LIF and Carcinoma
 Cardiovascular Diseases Antibodies  >164 publications with LIF and Cardiovascular Diseases
 Female Urogenital Diseases Antibodies  >127 publications with LIF and Female Urogenital Diseases
 Autoimmune Diseases Antibodies  >82 publications with LIF and Autoimmune Diseases
 Leukemia, Myeloid, Acute Antibodies  >67 publications with LIF and Leukemia, Myeloid, Acute
 Lung Diseases Antibodies  >59 publications with LIF and Lung Diseases

Organs/Tissues associated with anti-LIF antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >428 publications with LIF and Bone
 Brain Antibodies  >394 publications with LIF and Brain
 Embryonic Tissue Antibodies  >289 publications with LIF and Embryonic Tissue
 Muscle Antibodies  >288 publications with LIF and Muscle
 Uterus Antibodies  >286 publications with LIF and Uterus
 Bone Marrow Antibodies  >222 publications with LIF and Bone Marrow
 Skin Antibodies  >162 publications with LIF and Skin
 Lung Antibodies  >123 publications with LIF and Lung
 Placenta Antibodies  >120 publications with LIF and Placenta
 Eye Antibodies  >93 publications with LIF and Eye
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