Rabbit Anti-NT5E Polyclonal Antibody – Cat# MBS2006344

5'-Nucleotidase, Ecto (NT5E) Antibody – Rabbit Polyclonal

Name: NT5E Antibody; Polyclonal; Rabbit; Mouse/Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS2006344
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2006344

MBS2006344 is an antibody targeting NT5E. Also known as Biotin-Linked Antibody to 5'-Nucleotidase, Ecto (NT5E). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

5'-Nucleotidase, Ecto (NT5E), Polyclonal Antibody

Full Product Name

Biotin-Linked Antibody to 5'-Nucleotidase, Ecto (NT5E)

Product Gene Name

anti-NT5E antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, Human, Rat

Form/Format

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Purity/Purification

Antigen-specific Affinity Chromatography

Concentration

500ug/ml (lot specific)

Specificity

The antibody is a rabbit polyclonal antibody raised against NT5E conjugated to biotin. It has been selected for its ability to recognize NT5E in immunohistochemical staining andwestern blotting.

Preparation and Storage

Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Matching Pairs

Unconjugated Antibody: NT5E (MBS2002571)
Biotin Conjugated Antibody: NT5E (MBS2006344)

Matching Pairs

Biotin Conjugated Antibody: NT5E (MBS2006344)
Immunogen: NT5E (MBS2011862)

Chromosome Location

Chromosome: 6; NC_000006.11 (86159302..86205509). Location: 6q14-q21

OMIM

129190

3D Structure

ModBase 3D Structure for P21589

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Source

Antibody labeling

Label

Biotin

Traits

Liquid

Immunogen

Recombinant NT5E (Trp29~phe550) expressed in E.coli

Cross Reactivity

Mouse

Unconjugated Antibody

The unconjugated antibody version of this item is also available as catalog #MBS2002571

Product Categories/Family for anti-NT5E antibody

Biotin Antibody

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for anti-NT5E antibody

WB; ICC; IHC-P; IHC-F; IF; ELISA

Application Notes for anti-NT5E antibody

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

Optimal working dilutions must be determined by end user.

Other Notes

Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.

NCBI GI #

23897

NCBI GeneID

4907

NCBI Accession #

CAA39271.1

UniProt Primary Accession #

P21589

UniProt Secondary Accession #

O75520; Q5W116; B3KQI8

UniProt Related Accession #

P21589

NCBI Official Full Name

5'-nucleotidase

NCBI Official Synonym Full Names

5'-nucleotidase, ecto (CD73)

NCBI Official Symbol

NT5E

NCBI Official Synonym Symbols

NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

UniProt Protein Name

5'-nucleotidase

UniProt Synonym Protein Names

Ecto-5'-nucleotidase

UniProt Gene Name

NT5E

UniProt Synonym Gene Names

NT5; NTE; 5'-NT

UniProt Entry Name

5NTD_HUMAN

NCBI Protein Information

5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase

NCBI Summary for NT5E

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

UniProt Comments for NT5E

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Nucleotide Metabolism - purine; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

Research Articles on NT5E

1. This study demonstrated that the differential CD73 expression around intestinal tuberculosis granuloma indicates that Mycobacterium tuberculosis evades host immunity by recruiting mesenchymal cells with CD73 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NT5E antibody

Reference Product	PubMed Publications
ENPP1 antibody	>4 publications with NT5E and ENPP1
ADA antibody	>1 publications with NT5E and ADA
ENTPD1 antibody	>1 publications with NT5E and ENTPD1

Pathways associated with anti-NT5E antibody

Products by Pathway	Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Nicotinate And Nicotinamide Metabolism Pathway antibodies	Nicotinate And Nicotinamide Metabolism Pathway Diagram
Purine Catabolism Pathway antibodies	Purine Catabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Pyrimidine Catabolism Pathway antibodies	Pyrimidine Catabolism Pathway Diagram

Diseases associated with anti-NT5E antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>23 publications with NT5E and Neoplasms
Cardiovascular Diseases Antibodies	>16 publications with NT5E and Cardiovascular Diseases
Inflammation Antibodies	>15 publications with NT5E and Inflammation
Nervous System Diseases Antibodies	>9 publications with NT5E and Nervous System Diseases
Breast Neoplasms Antibodies	>8 publications with NT5E and Breast Neoplasms
Necrosis Antibodies	>7 publications with NT5E and Necrosis
Pain Antibodies	>7 publications with NT5E and Pain
Disease Models, Animal Antibodies	>6 publications with NT5E and Disease Models, Animal
Kidney Diseases Antibodies	>2 publications with NT5E and Kidney Diseases
Ovarian Neoplasms Antibodies	>2 publications with NT5E and Ovarian Neoplasms

Organs/Tissues associated with anti-NT5E antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>24 publications with NT5E and Blood
Vascular Antibodies	>15 publications with NT5E and Vascular
Bone Antibodies	>9 publications with NT5E and Bone
Brain Antibodies	>8 publications with NT5E and Brain
Muscle Antibodies	>7 publications with NT5E and Muscle
Ganglia Antibodies	>6 publications with NT5E and Ganglia
Mouth Antibodies	>6 publications with NT5E and Mouth
Embryonic Tissue Antibodies	>5 publications with NT5E and Embryonic Tissue
Bone Marrow Antibodies	>5 publications with NT5E and Bone Marrow
Skin Antibodies	>4 publications with NT5E and Skin