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Carbonic Anhydrase Vb, Mitochondrial (CA5B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2004341
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2004341 is an antibody targeting CA5B. Also known as Polyclonal Antibody to Carbonic Anhydrase VB, Mitochondrial (CA5B). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carbonic Anhydrase VB, Mitochondrial (CA5B), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Carbonic Anhydrase VB, Mitochondrial (CA5B)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-RQSPIN IR WRDSVYDPGL KPLTISYDPA TCLHIWNNGY SFLVEFEDST DKSVVEGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCY PAELHLVHWN AVKFESFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL PSIKHKDTLV EFGSFDPSCL MPTCP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against CA5B. It has been selected for its ability to recognize CA5B in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: CA5B (MBS2004341)
Biotin Conjugated Antibody: CA5B (MBS2001383)
Matching Pairs
Unconjugated Antibody: CA5B (MBS2004341)
Immunogen: CA5B (MBS2012151)
Molecular Weight
36,623 Da
3D Structure
ModBase 3D Structure for Q9QZA0
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant CA5B (Arg63~Pro225) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069808
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-CA5B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-CA5B antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-CA5B antibody

Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1.5ug/ml Rabbit Anti-Mouse CA5B Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
Rabbit Anti-CA5B Polyclonal Antibody – Cat# MBS2004341

Western Blot (WB) of anti-CA5B antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-CA5B Polyclonal Antibody, Western Blot (WB) – Cat# MBS2004341

Immunohistochemistry (IHC) of anti-CA5B antibody

DAB staining on fromalin fixed paraffin- embedded testis tissue)
Rabbit Anti-CA5B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2004341

Immunohistochemistry (IHC) of anti-CA5B antibody

DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue)
Rabbit Anti-CA5B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2004341

Other Notes

Small volumes of anti-CA5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CA5B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carbonic anhydrase 5B, mitochondrial
NCBI Official Synonym Full Names
carbonic anhydrase 5b, mitochondrial
NCBI Official Symbol
Car5b
NCBI Official Synonym Symbols
CAVB; Ca5b; CarVb; 7330410H16Rik; D730005F19Rik
UniProt Protein Name
Carbonic anhydrase 5B, mitochondrial
UniProt Synonym Protein Names
Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB
Protein Family
UniProt Gene Name
Ca5b
UniProt Synonym Gene Names
Car5b; CA-VB
NCBI Protein Information
carbonic anhydrase 5B, mitochondrial

UniProt Comments for CA5B

CA5B: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: X|X F5

Cellular Component: mitochondrion

Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Biological Process: one-carbon metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CA5B antibody

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