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SLC25A28 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9211847

MFRN2 is mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably… More details

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Product Overview

Online Datasheet in PDF
Product Name
MFRN2 (SLC25A28), Polyclonal Antibody
Full Product Name
MFRN2 Antibody (N-term)
Product Gene Name
anti-SLC25A28 antibody
Product Synonym Names
Mitoferrin-2; Mitochondrial RNA-splicing protein 3/4 homolog; MRS3/4; hMRS3/4; Mitochondrial iron transporter 2; Solute carrier family 25 member 28; SLC25A28; MFRN2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Predicted: Bovine, Canine, Rabbit
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This MFRN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human MFRN2.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
MFRN2 peptide (MBS9225581) is used for blocking the activity of MFRN2 antibody (MBS9211847)
Molecular Weight
Calculated MW: 39272
Immunogen Sequence Positions
36-64
OMIM
609767
3D Structure
ModBase 3D Structure for Q96A46
Cellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Tissue Location
Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart
Function
Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non erythroid cells

Product Categories/Family for anti-SLC25A28 antibody

Signal Transduction
Applications Tested/Suitable for anti-SLC25A28 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC)
Application Notes for anti-SLC25A28 antibody
WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50!!E~~Use at an assay dependent concentration

Western Blot (WB) of anti-SLC25A28 antibody

Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.
Rabbit Anti-SLC25A28 Polyclonal Antibody – Cat# MBS9211847

Western Blot (WB) of anti-SLC25A28 antibody

Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-SLC25A28 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9211847

Immunohistochemistry (IHC) of anti-SLC25A28 antibody

MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-SLC25A28 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9211847

Flow Cytometry (FC) of anti-SLC25A28 antibody

MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SLC25A28 Polyclonal Antibody, Flow Cytometry (FC) – Cat# MBS9211847

Product References and Citations for anti-SLC25A28 antibody

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Deloukas, P., et al. Nature 429(6990):375-381(2004)
Wistow, G., et al. Mol. Vis. 8, 185-195 (2002)

Other Notes

Small volumes of anti-SLC25A28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC25A28 purchase

MBS9211847 is an antibody targeting SLC25A28. Also known as MFRN2 Antibody (N-term). Reactive in Human
Predicted: Bovine, Canine, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC25A28. It may not necessarily be applicable to this product.

NCBI GI #
149274643
NCBI GeneID
81894
NCBI Accession #
NP_112489.3
UniProt Primary Accession #
Q96A46
UniProt Secondary Accession #
Q4VBZ0; Q5T777; Q86VX5; Q969G8; Q9H2J3
UniProt Related Accession #
Q96A46
GenBank Nucleotide #
NM_031212.3
NCBI Official Full Name
mitoferrin-2
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial iron transporter), member 28
NCBI Official Symbol
SLC25A28
NCBI Official Synonym Symbols
MFRN2; MRS4L; MRS3/4; NPD016
UniProt Protein Name
Mitoferrin-2
UniProt Synonym Protein Names
Mitochondrial RNA-splicing protein 3/4 homolog; MRS3/4; hMRS3/4; Mitochondrial iron transporter 2; Solute carrier family 25 member 28
Protein Family
Mitoferrin
UniProt Gene Name
SLC25A28
UniProt Synonym Gene Names
MFRN2; MRS3/4; hMRS3/4
UniProt Entry Name
MFRN2_HUMAN
NCBI Protein Information
mitoferrin-2

UniProt Comments for SLC25A28

SLC25A28: Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Mitochondrial

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: iron ion homeostasis; ion transport
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Research Articles on SLC25A28

1. Compares and contrasts all the known human SLC25A* genes and includes functional information.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SLC25A28 antibody

 Products by Pathway  Pathway Diagram
 Thiamine Metabolism Pathway antibodies  Thiamine Metabolism Pathway Diagram
 Thiamine Metabolism Pathway antibodies  Thiamine Metabolism Pathway Diagram

Diseases associated with anti-SLC25A28 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >2 publications with SLC25A28 and Anemia
 Paralysis Antibodies  >1 publications with SLC25A28 and Paralysis
 Uterine Cervical Neoplasms Antibodies  >1 publications with SLC25A28 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-SLC25A28 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2 publications with SLC25A28 and Heart
 Blood Antibodies  >1 publications with SLC25A28 and Blood
 Kidney Antibodies  >1 publications with SLC25A28 and Kidney
 Liver Antibodies  >1 publications with SLC25A28 and Liver
 Testis Antibodies  >1 publications with SLC25A28 and Testis
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