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OxiSelect In Vitro ROS/RNS Assay Kit

Scan QR to view Datasheet Catalog #    MBS168257
Unit / Price
20 Assays  /  $320 +1 FREE 8GB USB
96 Assays  /  $585 +1 FREE 8GB USB
480 Assays  /  $2,255 +2 FREE 8GB USB
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 Product Name   

OxiSelect In Vitro ROS/RNS, Assay Kit

★Popular Item★
 Also Known As   

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Preparation and Storage    Upon receipt, store the DCF-DiOxyQ and DCF Standard at -20 degree C. Avoid multiple freeze/thaw cycles. Store all other components at 4 degree C.
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OxiSelect In Vitro ROS/RNS assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for OxiSelect In Vitro ROS/RNS assay kit

   Principle of the Assay: The OxiSelect In Vitro ROS/RNS Assay Kit is an in vitro assay for measuring total ROS/RNS free radical activity. Unknown ROS or RNS samples or standards are added to the wells with a catalyst that helps accelerate the oxidative reaction. After a brief incubation, the prepared DCFH probe is added to all wells and the oxidation reaction is allowed to proceed (Figure 1). Samples are measured fluorometrically against a hydrogen peroxide or DCF standard. The assay is performed in a 96-well fluorescence plate format that can be read on a standard fluorescence plate reader. The free radical content in unknown samples is determined by comparison with the predetermined DCF or hydrogen peroxide standard curve.

Background/Introduction: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are well-established molecules responsible for the deleterious effects of oxidative stress. Accumulation of free radicals coupled with an increase in oxidative stress has been implicated in the pathogenesis of several disease states. The role of oxidative stress in vascular diseases, diabetes, renal ischemia, atherosclerosis, pulmonary pathological states, inflammatory diseases, cancer, as well as ageing has been well established. Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins and lipids. Measuring the effect of antioxidant therapies and ROS/RNS activity is crucial to suppressing or treating oxidative stress inducers. The OxiSelect In Vitro ROS/RNS Assay Kit is an assay for measuring the total free radical presence of a sample. The assay employs a proprietary quenched fluorogenic probe, dichlorodihydrofluorescin DiOxyQ (DCFH-DiOxyQ), which is a specific ROS/RNS probe that is based on similar chemistry to the popular 2', 7'-dichlorodihydrofluorescein diacetate. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2', 7'-dichlorodihydrofluorescein (DCF) (Figure 1). Fluorescence intensity is proportional to the total ROS/RNS levels within the sample. The DCFH-DiOxyQ probe can react with hydrogen peroxide (H2O2), peroxyl radical (ROO?), nitric oxide (NO), and peroxynitrite anion (ONOO-). These free radical molecules are representative of both ROS and RNS, thus allowing for measurement of the total free radical population within a sample. OxiSelect In Vitro ROS/RNS Assay Kit can also be used to evaluate antioxidant's effect on free radicals. The kit has a detection sensitivity limit of 10 pM for DCF and 40 nM for H2O2 respectively. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
 Product Categories/Family for OxiSelect In Vitro ROS/RNS assay kit    Oxidative Stress/ Damage; Reactive Oxygen Species (ROS) Assays; In Vitro ROS/RNS Assay
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 Testing Data of OxiSelect In Vitro ROS/RNS assay kit    OxiSelect In Vitro ROS/RNS assay kit Testing Data image
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 Testing Data #2 of OxiSelect In Vitro ROS/RNS assay kit    OxiSelect In Vitro ROS/RNS assay kit Testing Data #2 image
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Sample Manual Insert of MBS168257. Click to request current manual
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Product References and Citations for OxiSelect In Vitro ROS/RNS assay kit

   1. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J Immunol. 1983; 130:1910-1917.
2. Brandt R, Keston AS. Synthesis of diacetyldichlorofluorescin: A stable reagent for fluorometric analysis. Anal Biochem. 1965; 11:6-9.
3. Keston AS, Brandt R. The fluorometric analysis of ultramicro quantities of hydrogen peroxide. Anal Biochem.1965; 11:1-5.
1. Schmidt-Heydt, M. et al. (2015). Oxidative stress induces the biosynthesis of citrinin by penicillium verrucosum at the expense of ochratoxin. Int J Food Microbiol. 192:1-6.
2. Khadir, A. et al. (2015). MAP kinase phosphatase DUSP1 is overexpressed in obese humans and modulated by physical exercise. Am J Physiol Endocrinol Metab. 308:E71-E83.
3. Ravassa, S. et al. (2015). Association of low GLP-1 with oxidative stress is related to cardiac disease and outcome in patients with type 2 diabetes mellitus: a pilot study. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2015.01.002.
4. Canivet, L. et al. (2015). Effects of engineered iron nanoparticles on the bryophyte, Physcomitrella patens (Hedw.) Bruch & Schimp, after foliar exposure. Ecotoxicol Environ Saf. 113:499-505.
5. Meenalochani, S. et al. (2015). Sphingosine kinase 2 and sphingosine-1-phosphate promotes mitochondrial function in dopaminergic neurons of mouse model of Parkinson's Disease and in MPP+-treated MN9D cells in vitro. Neuroscience. doi: 10.1016/j.neuroscience.2015.01.032.
6. Walsh, C. J. et al. (2015). Sublethal red tide toxin exposure in free-ranging manatees (Trichechus manatus) affects the immune system through reduced lymphocyte proliferation responses, inflammation, and oxidative stress. Aquatic Toxicology. 161:73-84.
7. Rhyu, H. S. et al. (2014). The effects of ketogenic diet on oxidative stress and antioxidative capacity markers of Taekwondo athletes. J Exerc Rehabil. 10:362-366.
8. Hao, Y. et al. (2014). Mycoplasma pneumoniae modulates STAT3-STAT6/EGFR-FOXA2 signaling to induce overexpression of airway mucins. Infect Immun. 82:5246-5255.
9. Pandey, D. et al. (2014). Transcriptional regulation of endothelial arginase 2 by histone deacetylase 2. Arterioscler Thromb Vasc Biol. 34:1556-1566.
10. Liu, X. et al. (2013). Epoxyeicosatrienoic acids prevent cisplatin-induced renal apoptosis through a p38 mitogen-activated protein kinase-regulated mitochondrial pathway. Mol. Pharmacol. 84:925-934.
11. Song, J. et al. (2013). Nicotinamide phosphoribosyltransferase is required for the calorie restriction-mediated improvements in oxidative stress, mitochondrial biogenesis, and metabolic adaptation. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glt122.
12. Xiao, D. et al. (2013). Estrogen normalizes perinatal nicotine-induced hypertensive responses in adult female rat offspring. Hypertension. 61:1246-1254.
13. Wang, W. et al. (2012). Mono-(2-ethylhexyl) phthalate induces oxidative stress and inhibits growth of mouse ovarian antral follicles. Biol Reprod. 87:152.
14. Ju, D.J. et al. (2012). Ethyl pyruvate ameliorates albuminuria and glomerular injury in the animal model of diabetic nephropathy. Am J Physiol Renal Physiol. 302: F606-F613.
15. Momi, S. et al. (2012). Nitric oxide enhances the anti-inflammatory and anti-atherogenic activity of atorvastatin in a mouse model of accelerated atherosclerosis. Cardiovasc Res. 10.1093/cvr/cvs100.
16. Patterson, A.J. et al. (2011). Hypoxia-derived oxidative stress mediates epigenetic repression of pkc? gene in foetal rat hearts. Cardiovasc Res. 10.1093/cvr/cvr322.
17. Rathnasamy, G. et al. (2011). Iron and iron regulatory proteins in amoeboid microglial cells are linked to oligodendrocyte death in hypoxic neonatal rat periventricular white matter through production of proinflammatory cytokines and reactive oxygen/nitrogen species. J. Neurosci. 31:17982-17995.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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