Triiodothyronine (T3) ELISA Kit for General

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of T3 in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to T3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled T3 and unlabeled T3 (Standards or samples) with the pre-coated antibody specific to T3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of T3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of T3 in the sample.

Effect of Quercetin Nanoparticles on Hepatic and Intestinal Enzymes and Stress-Related Genes in Nile Tilapia Fish Exposed to Silver Nanoparticles; Biomedicines; 2023 Feb 22;11(3):663.; PMID: 36979642; Mayada R Farag; Forensic Medicine and Toxicology Department, Veterinary Medicine Faculty, Zagazig University, Zagazig 44519, Egypt.

Small volumes of T3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2700145 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Triiodothyronine (T3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing T3. The ELISA analytical biochemical technique of the MBS2700145 kit is based on T3 antibody-T3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect T3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, T3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).