Noradrenaline (NE) ELISA Kit for General

Name: General NE ELISA Kit; Noradrenaline; MyBioSource
Brand: MyBioSource
SKU: MBS2700394
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2700394

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to noradrenaline has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled… More details

$320 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Noradrenaline (NE), ELISA Kit

Full Product Name

General Noradrenaline (NE) ELISA Kit

Product Gene Name

NE elisa kit

Product Synonym Names

NA; NAd; Norepinephrine; Norepi

Species Reactivity

General

Assay Type

Quantitative Competitive

Samples

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

61.7-5,000pg/mL

Sensitivity

< 24.4pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of noradrenaline. No significant cross-reactivity or interference between noradrenaline and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level noradrenaline were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level noradrenaline were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

57,370 Da

OMIM

M65105 mRNA

3D Structure

ModBase 3D Structure for P23975

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Other Notes

Small volumes of NE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NE purchase

MBS2700394 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Noradrenaline (NE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NE. The ELISA analytical biochemical technique of the MBS2700394 kit is based on NE antibody-NE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NE. It may not necessarily be applicable to this product.

NCBI GI #

189258

NCBI GeneID

6530

NCBI Accession #

AAA59943.1

UniProt Primary Accession #

P23975

UniProt Secondary Accession #

Q96KH8; B2R707; B4DX48

UniProt Related Accession #

P23975

NCBI Official Full Name

noradrenaline transporter

NCBI Official Synonym Full Names

solute carrier family 6 member 2

NCBI Official Symbol

SLC6A2

NCBI Official Synonym Symbols

NET; NAT1; NET1; SLC6A5

UniProt Protein Name

Sodium-dependent noradrenaline transporter

UniProt Synonym Protein Names

Norepinephrine transporter; NET; Solute carrier family 6 member 2

UniProt Gene Name

SLC6A2

UniProt Synonym Gene Names

NAT1; NET1; SLC6A5; NET

NCBI Protein Information

sodium-dependent noradrenaline transporter

NCBI Summary for NE

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

UniProt Comments for NE

Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.

Research Articles on NE

1. T allele of the SNP rs7194256 in the 3'UTR of the NET gene is more prevalent in diseases where NET impairment is evident, including essential hypertension, depression, panic disorder and postural orthostatic tachycardia syndrome; mechanism involved may include the creation of a binding site for the miRNA miR-19-3p, which is, at least in part, regulated by circulating norepinephrine concentrations

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NE elisa kit

Reference Product	PubMed Publications
SLC18A2 elisa kit	>12 publications with NE and SLC18A2
PICK1 elisa kit	>2 publications with NE and PICK1
STX1A elisa kit	>1 publications with NE and STX1A

Pathways associated with NE elisa kit

Products by Pathway	Pathway Diagram
Monoamine Transport Pathway antibodies	Monoamine Transport Pathway Diagram
Na+/Cl- Dependent Neurotransmitter Transporters Pathway antibodies	Na+/Cl- Dependent Neurotransmitter Transporters Pathway Diagram
SLC-mediated Transmembrane Transport Pathway antibodies	SLC-mediated Transmembrane Transport Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram
Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies	Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram

Diseases associated with NE elisa kit

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>120 publications with NE and Mental Disorders
Nervous System Diseases Antibodies	>49 publications with NE and Nervous System Diseases
Attention Deficit Disorder with Hyperactivity Antibodies	>49 publications with NE and Attention Deficit Disorder with Hyperactivity
Cardiovascular Diseases Antibodies	>47 publications with NE and Cardiovascular Diseases
Depressive Disorder Antibodies	>36 publications with NE and Depressive Disorder
Heart Diseases Antibodies	>32 publications with NE and Heart Diseases
Brain Diseases Antibodies	>30 publications with NE and Brain Diseases
Hypertension Antibodies	>26 publications with NE and Hypertension
Disease Models, Animal Antibodies	>25 publications with NE and Disease Models, Animal
Tachycardia Antibodies	>17 publications with NE and Tachycardia

Organs/Tissues associated with NE elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>276 publications with NE and Brain
Nerve Antibodies	>267 publications with NE and Nerve
Muscle Antibodies	>44 publications with NE and Muscle
Kidney Antibodies	>28 publications with NE and Kidney
Placenta Antibodies	>18 publications with NE and Placenta
Intestine Antibodies	>2 publications with NE and Intestine
Testis Antibodies	>2 publications with NE and Testis
Prostate Antibodies	>1 publications with NE and Prostate