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RSPO2 elisa kit :: Human R-Spondin 2 (RSPO2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024804 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

R-Spondin 2 (RSPO2), ELISA Kit

★Popular Item★
 Also Known As   

R-Spondin 2 (RSPO2) ELISA Kit

 Product Synonym Names    Roof plate-specific spondin-2
 Product Gene Name   

RSPO2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    610575
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of R-Spondin 2 (RSPO2).
No significant cross-reactivity or interference between R-Spondin 2 (RSPO2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    15.6-1000pg/mL
 Sensitivity    < 6.1pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RSPO2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RSPO2 purchase    MBS2024804 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the R-Spondin 2 (RSPO2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000pg/mL in biological research samples containing RSPO2, with an estimated sensitivity of < 6.1pg/mL. The ELISA analytical biochemical technique of the MBS2024804 kit is based on RSPO2 antibody-RSPO2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSPO2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSPO2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RSPO2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2 (RSPO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 2 (RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2 (RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 2 (RSPO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for RSPO2 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of RSPO2 elisa kit    RSPO2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024804. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RSPO2. It may not necessarily be applicable to this product.
 NCBI GI #    544583460
 NCBI GeneID    340419
 NCBI Accession #    NP_001269792.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001282863.1 [Other Products]
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 UniProt Secondary Accession #    Q4G0U4; Q8N6X6; B3KVP0 [Other Products]
 UniProt Related Accession #    Q6UXX9 [Other Products]
 Molecular Weight    20,972 Da
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 NCBI Official Full Name    R-spondin-2 isoform 2
 NCBI Official Synonym Full Names    R-spondin 2
 NCBI Official Symbol    RSPO2 [Similar Products]
 NCBI Official Synonym Symbols   
CRISTIN2
[Similar Products]
 NCBI Protein Information    R-spondin-2
 UniProt Protein Name    R-spondin-2
 UniProt Synonym Protein Names   
Roof plate-specific spondin-2; hRspo2
 Protein Family    R-spondin
 UniProt Gene Name    RSPO2 [Similar Products]
 UniProt Synonym Gene Names    hRspo2 [Similar Products]
 UniProt Entry Name    RSPO2_HUMAN
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 NCBI Summary for RSPO2    R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for RSPO2    R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: osteoblast differentiation; embryonic forelimb morphogenesis; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization
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 Research Articles on RSPO2    1. Wnt(high) cells were more likely to give rise to Wnt(high) progeny, tended to be more metastatic, and revealed higher levels of RSPO2 expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RSPO2 elisa kitPathways associated with RSPO2 elisa kit
 Reference Product  PubMed Publications
 PTPRK elisa kit  >3 publications with RSPO2 and PTPRK
 SP8 elisa kit  >2 publications with RSPO2 and SP8
 WNT11 elisa kit  >1 publications with RSPO2 and WNT11
 FGF5 elisa kit  >1 publications with RSPO2 and FGF5
 KRT71 elisa kit  >1 publications with RSPO2 and KRT71
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
 Regulation Of FZD By Ubiquitination Pathway antibodies  Regulation Of FZD By Ubiquitination Pathway Diagram
Diseases associated with RSPO2 elisa kitOrgans/Tissues associated with RSPO2 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >5 publications with RSPO2 and Carcinoma
 Adenocarcinoma Antibodies  >3 publications with RSPO2 and Adenocarcinoma
 Nervous System Diseases Antibodies  >3 publications with RSPO2 and Nervous System Diseases
 Hyperplasia Antibodies  >3 publications with RSPO2 and Hyperplasia
 Carcinoma, Hepatocellular Antibodies  >2 publications with RSPO2 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >2 publications with RSPO2 and Fibrosis
 Neoplasms, Experimental Antibodies  >1 publications with RSPO2 and Neoplasms, Experimental
 Abnormalities, Multiple Antibodies  >1 publications with RSPO2 and Abnormalities, Multiple
 Brain Diseases Antibodies  >1 publications with RSPO2 and Brain Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with RSPO2 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with RSPO2 and Lung
 Muscle Antibodies  >5 publications with RSPO2 and Muscle
 Intestine Antibodies  >4 publications with RSPO2 and Intestine
 Brain Antibodies  >4 publications with RSPO2 and Brain
 Blood Antibodies  >3 publications with RSPO2 and Blood
 Embryonic Tissue Antibodies  >2 publications with RSPO2 and Embryonic Tissue
 Liver Antibodies  >2 publications with RSPO2 and Liver
 Ear Antibodies  >1 publications with RSPO2 and Ear
 Skin Antibodies  >1 publications with RSPO2 and Skin
 Testis Antibodies  >1 publications with RSPO2 and Testis
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