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NPPC elisa kit :: Human natriuretic peptide precursor C ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704887
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
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 Product Name   

natriuretic peptide precursor C (NPPC), ELISA Kit

 Also Known As   

Human C -type natriuretic peptide, CNP ELISA Kit

 Product Synonym Names    Human C -type natriuretic peptide; CNP ELISA Kit; CNP; OTTHUMP00000203815; natriuretic peptide precursor C
 Product Gene Name   

NPPC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704887 Testing Data    Testing Data PDF
 OMIM    600296
 3D Structure    ModBase 3D Structure for P23582
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human NPPC. No significant cross-reactivity or interference between Human NPPC and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates
 Detection Range    62.5 pg/ml-4000 pg/ml
 Sensitivity    15.6 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Developmental Protein
 Protein Biological Process 3    Osteogenesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPPC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPPC purchase    MBS704887 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the natriuretic peptide precursor C (NPPC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing NPPC, with an estimated sensitivity of 15.6 pg/ml. The ELISA analytical biochemical technique of the MBS704887 kit is based on NPPC antibody-NPPC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPPC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPPC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NPPC elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CNP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NPPC elisa kit    NPPC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704887. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPPC. It may not necessarily be applicable to this product.
 NCBI GI #    13249346
 NCBI GeneID    4880
 NCBI Accession #    NP_077720.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024409.2 [Other Products]
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 UniProt Primary Accession #    P23582 [Other Products]
 UniProt Secondary Accession #    Q4ZG41 [Other Products]
 UniProt Related Accession #    P23582 [Other Products]
 Molecular Weight    13,246 Da
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 NCBI Official Full Name    C-type natriuretic peptide
 NCBI Official Synonym Full Names    natriuretic peptide C
 NCBI Official Symbol    NPPC [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    C-type natriuretic peptide; natriuretic peptide precursor C
 UniProt Protein Name    C-type natriuretic peptide
 Protein Family    C-type natriuretic peptide
 UniProt Gene Name    NPPC [Similar Products]
 UniProt Synonym Gene Names    CNP2 [Similar Products]
 UniProt Entry Name    ANFC_HUMAN
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 NCBI Summary for NPPC    The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]
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 UniProt Comments for NPPC    Function: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3 1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on NPPC    1. Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NPPC elisa kitDiseases associated with NPPC elisa kit
 Reference Product  PubMed Publications
 NPR2 elisa kit  >20 publications with NPPC and NPR2
 NPPA elisa kit  >8 publications with NPPC and NPPA
 NPR3 elisa kit  >7 publications with NPPC and NPR3
 NPPB elisa kit  >6 publications with NPPC and NPPB
 NPR1 elisa kit  >4 publications with NPPC and NPR1
 Disease Name  Pubmed Publications
 Bone Diseases, Developmental Antibodies  >7 publications with NPPC and Bone Diseases, Developmental
 Nervous System Diseases Antibodies  >5 publications with NPPC and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >4 publications with NPPC and Cardiovascular Diseases
 Growth Disorders Antibodies  >4 publications with NPPC and Growth Disorders
 Hypertension Antibodies  >3 publications with NPPC and Hypertension
 Disease Models, Animal Antibodies  >3 publications with NPPC and Disease Models, Animal
 Heart Diseases Antibodies  >3 publications with NPPC and Heart Diseases
 Cartilage Diseases Antibodies  >3 publications with NPPC and Cartilage Diseases
 Hypertrophy Antibodies  >2 publications with NPPC and Hypertrophy
 Demyelinating Diseases Antibodies  >1 publications with NPPC and Demyelinating Diseases
Organs/Tissues associated with NPPC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with NPPC and Brain
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