Uroplakin-3a (UPK3A) ELISA Kit for Mouse

Name: Mouse UPK3A ELISA Kit; Uroplakin-3a; MyBioSource
Brand: MyBioSource
SKU: MBS285309
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285309

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate UPK3A in samples. An antibody specific for UPK3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPK3A present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Uroplakin-3a (UPK3A), ELISA Kit

Full Product Name

Mouse Uroplakin-3a (UPK3A) ELISA Kit

Product Gene Name

UPK3A elisa kit

Product Synonym Names

CTA-268H5.10; MGC119178; UP3A; UPIII; UPIIIA; UPK3; uroplakin III

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse UPK3A. No significant cross-reactivity or interference between Mouse UPK3A and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

30,990 Da

3D Structure

ModBase 3D Structure for Q9JKX8

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of UPK3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UPK3A purchase

MBS285309 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Uroplakin-3a (UPK3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UPK3A. The ELISA analytical biochemical technique of the MBS285309 kit is based on UPK3A antibody-UPK3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UPK3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UPK3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for UPK3A. It may not necessarily be applicable to this product.

NCBI GI #

251823972

NCBI GeneID

22270

NCBI Accession #

NP_075967.2

UniProt Primary Accession #

Q9JKX8

UniProt Secondary Accession #

Q27QV3

UniProt Related Accession #

Q9JKX8

GenBank Nucleotide #

NM_023478.2

NCBI Official Full Name

uroplakin-3a

NCBI Official Synonym Full Names

uroplakin 3A

NCBI Official Symbol

Upk3a

NCBI Official Synonym Symbols

UP3a; Upk3; UPIII; 1110017C07Rik

UniProt Protein Name

Uroplakin-3a

UniProt Synonym Protein Names

Uroplakin III; UPIII

Protein Family

Uroplakin

UniProt Gene Name

Upk3a

UniProt Synonym Gene Names

Upk3; UP3a; UPIII

NCBI Protein Information

uroplakin-3a

UniProt Comments for UPK3A

UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15|15 E2

Cellular Component: apical plasma membrane; endoplasmic reticulum; integral component of membrane; membrane

Molecular Function: protein binding

Biological Process: cell morphogenesis; epithelial cell differentiation; kidney development; potassium ion homeostasis; sodium ion homeostasis; urea transport; urinary bladder development; water transport

Research Articles on UPK3A

1. U-CCs (Uroplakin3a expressing cells )proliferate and generate CCs and ciliated cells in uninjured airways long-term and, like v-CCs, after Nap. U-CCs have a higher propensity to generate ciliated cells than non-U-CCs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with UPK3A elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>13 publications with UPK3A and Carcinoma
Urinary Bladder Neoplasms Antibodies	>10 publications with UPK3A and Urinary Bladder Neoplasms
Carcinoma, Transitional Cell Antibodies	>8 publications with UPK3A and Carcinoma, Transitional Cell
Neoplasm Metastasis Antibodies	>4 publications with UPK3A and Neoplasm Metastasis
Kidney Diseases Antibodies	>3 publications with UPK3A and Kidney Diseases
Adenocarcinoma Antibodies	>3 publications with UPK3A and Adenocarcinoma
Cystitis Antibodies	>3 publications with UPK3A and Cystitis
Ovarian Neoplasms Antibodies	>2 publications with UPK3A and Ovarian Neoplasms
Recurrence Antibodies	>2 publications with UPK3A and Recurrence
Cell Transformation, Neoplastic Antibodies	>2 publications with UPK3A and Cell Transformation, Neoplastic

Organs/Tissues associated with UPK3A elisa kit

Organ/Tissue Name	Pubmed Publications
Bladder Antibodies	>24 publications with UPK3A and Bladder
Lung Antibodies	>1 publications with UPK3A and Lung
Embryonic Tissue Antibodies	>1 publications with UPK3A and Embryonic Tissue