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Interferon Beta (IFNb) ELISA Kit for Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025528
SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb). Standards or samples are then added to the appropriate microtiter plate… More details

$420 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interferon Beta (IFNb), ELISA Kit
Full Product Name
Rabbit Interferon Beta (IFNb) ELISA Kit
Product Gene Name
IFNb elisa kit
Product Synonym Names
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
Species Reactivity
Rabbit
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
6.0pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb).
No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
22,294 Da
OMIM
147640
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%

Product Categories/Family for IFNb elisa kit

Cytokine; Tumor immunity; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of IFNb elisa kit

Rabbit IFNb ELISA Kit – Cat# MBS2025528

Other Notes

Small volumes of IFNb elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFNb purchase

MBS2025528 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon Beta (IFNb) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFNb. The ELISA analytical biochemical technique of the MBS2025528 kit is based on IFNb antibody-IFNb antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNb antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNb. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IFNb. It may not necessarily be applicable to this product.

NCBI GI #
4504603
NCBI GeneID
3456
NCBI Accession #
NP_002167.1
UniProt Secondary Accession #
Q5VWC9
UniProt Related Accession #
P01574
GenBank Nucleotide #
NM_002176.2
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
UniProt Protein Name
Interferon beta
UniProt Synonym Protein Names
Fibroblast interferon
Protein Family
Interferon
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN
NCBI Protein Information
interferon beta

UniProt Comments for IFNb

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response
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Research Articles on IFNb

1. Applied an affinity proteomic approach and identified ELAVL1/Hu antigen R (HuR) as the predominant RNA-binding protein of the IFN-beta mRNA (A/U)-rich elements (AREs).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IFNb elisa kit

 Reference Product  PubMed Publications
 IRF3 elisa kit  >19 publications with IFNb and IRF3
 IFNAR1 elisa kit  >6 publications with IFNb and IFNAR1
 STAT1 elisa kit  >5 publications with IFNb and STAT1
 IRF7 elisa kit  >5 publications with IFNb and IRF7
 STAT2 elisa kit  >3 publications with IFNb and STAT2
 IRF1 elisa kit  >1 publications with IFNb and IRF1
 IFNAR2 elisa kit  >1 publications with IFNb and IFNAR2
 SOCS3 elisa kit  >1 publications with IFNb and SOCS3

Pathways associated with IFNb elisa kit

 Products by Pathway  Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram

Diseases associated with IFNb elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with IFNb and Inflammation
 Immune System Diseases Antibodies  >9 publications with IFNb and Immune System Diseases
 Necrosis Antibodies  >7 publications with IFNb and Necrosis
 Lung Diseases Antibodies  >7 publications with IFNb and Lung Diseases
 Carcinoma Antibodies  >5 publications with IFNb and Carcinoma
 Melanoma Antibodies  >3 publications with IFNb and Melanoma
 Lung Neoplasms Antibodies  >3 publications with IFNb and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with IFNb and Neoplasms, Experimental
 Multiple Sclerosis Antibodies  >2 publications with IFNb and Multiple Sclerosis
 Pancreatic Neoplasms Antibodies  >2 publications with IFNb and Pancreatic Neoplasms
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