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Complement Component 5a (C5A) Rattus Norvegicus (Rat) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2011706
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2011706 is a recombinant protein targeting C5a. Also known as Recombinant Complement Component 5a (C5a). Reactive in Rattus norvegicus (Rat). Host: E.coli. More details

$200 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Complement Component 5a (C5a), Recombinant Protein
Full Product Name
Recombinant Complement Component 5a (C5a)
Product Gene Name
C5a recombinant protein
Host
E.coli
Species Reactivity
Rattus norvegicus (Rat)
Form/Format
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin 300.
Purity/Purification
> 90%
Concentration
Original Concentration: 200ug/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: C5a (MBS2006523)
Immunogen: C5a (MBS2011706)
Matching Pairs
APC-CY7 Conjugated Antibody: C5a (MBS2041764)
Immunogen: C5a (MBS2011706)
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Molecular Weight
Predicted Molecular Mass: 36.1kDa
Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
Chromosome Location
Chromosome: 3; NC_005102.3 (19369981..19432948, complement). Location: 3p11
3D Structure
ModBase 3D Structure for P08650
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Prokaryotic expression
Residues
Gln12~Ile64
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
8.0
Usage
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for C5a recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes for C5a recombinant protein
(May be suitable for use in other assays to be determined by the end user.)

Sequence of C5a recombinant protein

Rattus Norvegicus (Rat) C5A Recombinant Protein – Cat# MBS2011706

SDS-Page of C5a recombinant protein

Rattus Norvegicus (Rat) C5A Recombinant Protein, SDS-Page – Cat# MBS2011706

Gene Sequencing (extract) of C5a recombinant protein

Rattus Norvegicus (Rat) C5A Recombinant Protein, Gene Sequencing (extract) – Cat# MBS2011706

Other Notes

Small volumes of C5a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for C5a. It may not necessarily be applicable to this product.

NCBI GI #
46397770
NCBI GeneID
362119
NCBI Accession #
P08650.2
UniProt Primary Accession #
P08650
UniProt Secondary Accession #
Q63078
UniProt Related Accession #
P08650
NCBI Official Full Name
Complement C5
NCBI Official Synonym Full Names
complement component 5
NCBI Official Symbol
C5
NCBI Official Synonym Symbols
Hc; C5a; RGD1561905
UniProt Protein Name
Complement C5
UniProt Gene Name
C5
UniProt Entry Name
CO5_RAT
NCBI Protein Information
complement C5; anaphylatoxin

NCBI Summary for C5a

likely component of the classical pathway of the complement cascade; may be involved with tissue injury associated with ischemia or sepsis; may be a component of the membrane attack complex [RGD, Feb 2006]

UniProt Comments for C5a

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space

Molecular Function: C5a anaphylatoxin chemotactic receptor binding

Biological Process: cellular calcium ion homeostasis; positive regulation of angiogenesis; positive regulation of chemotaxis; in utero embryonic development; complement activation, alternative pathway; chemotaxis; glucose homeostasis; inflammatory response; negative regulation of dopamine secretion; complement activation, classical pathway; leukocyte migration during inflammatory response
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Research Articles on C5a

1. Early immune-inflammatory response to blast injury involves complement and TNFalpha, which correlates with hippocampus and cerebral cortex damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with C5a recombinant protein

 Products by Pathway  Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram

Organs/Tissues associated with C5a recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >438 publications with C5a and Kidney
 Liver Antibodies  >416 publications with C5a and Liver
 Embryonic Tissue Antibodies  >63 publications with C5a and Embryonic Tissue
 Adrenal Gland Antibodies  >10 publications with C5a and Adrenal Gland
 Uncharacterized Tissue Antibodies  >2 publications with C5a and Uncharacterized Tissue
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