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SDS-PAGE Sample Loading Buffer (6x)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355534

Background/Introduction: SDS-PAGE Sample Loading Buffer is a kind of 6 × concentrated solution which based on Bromophenol blue dye. This reagent contains SDS, DTT, Glycerol, Bromophenol blue, Tris-HCL buffer, ect. It can be used for common SDS?PAGE protein… More details

$215 / 5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SDS-PAGE Sample Loading Buffer (6x), Buffer
Full Product Name
SDS-PAGE Sample Loading Buffer (6x)
Protocol
  1. Dissolve the SDS-PAGE Sample Loading Buffer, 6 x in water bath at room temperature or not exceeding 37°C. After dissolving, store at room temperature immediately.
  2. Mix sample with SDS-PAGE Sample Loading Buffer, 6 x at the ratio 5:1 (i.e. add 5ul sample into 1ul of SDS-PAGE Sample Loading Buffer)
  3. Fully denaturize sample at 100°C or boiling water bath for 3-5 min
  4. Cool the sample to room temperature, then load and electrophorese.
  5. Stop electrophoresis when Bromophenol blue dye arrives at the bottom of gels.
Notes
  1. If SDS sediments appears when balance SDS-PAGE Sample Loading Buffer to room temperature, try to melt it with warm water.
  2. The slightly sharp smell may encountered for the few amount of DTT
  3. Please operate with gloves in the fume hood.

Product Categories/Family for SDS-PAGE Sample Loading Buffer (6x) buffer

Protein Electrophoresis

Other Notes

Small volumes of SDS-PAGE Sample Loading Buffer (6x) buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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MBS355534 is a buffer targeting SDS-PAGE Sample Loading Buffer (6x).
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